Preparation and Distribution of DOC Consensus Reference Materials 2022-2025

世界海洋中的溶解有机碳（DOC）是地球上活跃循环碳的主要储库之一。DOC所含的碳量相当于所有大陆生物总量的总和。了解这个碳库的运动和转变对了解地球的碳循环至关重要。测量和跟踪这一巨大的碳库及其动态的能力需要全球数十个海洋实验室之间的密切合作。确保所有这些实验室都在进行准确的DOC测量，需要一种方法来确认他们的分析是一致的，并与其他实验室相比较。该奖项为一个计划提供支持，该计划准备和分发由国际共识制定的海水DOC浓度标准。迈阿密大学将继续一个共识参考材料（CRM）项目，该项目最初是在1998年开发的，旨在支持国际海洋化学团体对溶解有机碳进行一致、高质量的测量。随着这项工作的继续，美国和国际社会将能够继续对一个主要的海洋碳库进行可靠的测量，从而提高社会评估海洋碳循环变化和海洋在气候变化中的作用的能力。该计划为学生和首席研究员实验室的技术人员提供培训机会，并支持他的教学和服务工作。随着国际上对海洋碳循环研究的持续努力，现有的CRM计划为全球可比的海洋DOC测量提供了必要的基准。来自佛罗里达海峡深处的水（~42 mol/kg DOC）将被收集，放入安瓿中，并分发给美国和国际社会。自项目启动以来，已向45个国家的300个美国和国际实验室分发了374,000瓶参考水，在21年度以每年约27,000瓶的速度分发。随着该计划为期3年的支持期的延长，来自海洋上层（SSR）、中层（MSR）和深层（DSR）以及低碳参考水域（LCW）的参考材料的分发，支持了DOC社区的分析工作。此外，在先前授予期间开发的新产品将继续进行：DOC-TN测定试剂盒。该试剂盒配备了所有必要的分析样品，以确定DOC和总氮（TN），包括4个碳和氮的校准标准，和4个参考物质（DSR， MSR， SSR和LCW）。它还包括Excel模板，用于指导每次分析运行的标准，crm和样品的顺序。该试剂盒满足那些谁正在寻求建立自己的分析能力，以下指导方针提供了由PI实验室的需求。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Preparation and Distribution of DOC Consensus Reference Materials 2022-2025Dissolved organic carbon (DOC) in the world ocean is one of the major pools of actively cycled carbon on Earth. DOC holds as much carbon as the living biomass on all continents combined. It is vital to understand the movement and transformations of this carbon pool to understand the Earth’s carbon cycle. The ability to measure and track this large pool of carbon and its dynamics requires close collaboration between dozens of marine laboratories worldwide. Ensuring that all these laboratories are making accurate DOC measurements requires a way to confirm that their analyses are consistent and comparable to the other laboratories. This award provides support for a program that prepares and distributes a seawater standard for DOC concentration that is set by international consensus. The University of Miami will continue a consensus reference material (CRM) program that was first developed in 1998 in support of consistent, high-quality measurements of dissolved organic carbon by the international ocean chemistry community. With continuation of the work, the US and international communities will be able to continue to make reliable measurements of a major marine carbon reservoir, thereby advancing society’s ability to assess changes in the marine carbon cycle and the role of the ocean in climate change. This program provides training opportunities for students and technicians in the lead investigator’s lab and supports his teaching and service work.With continued strong international efforts to investigate the marine carbon cycle, the existing CRM program provides the necessary benchmark for globally comparable marine DOC measurements. Water from the deep Florida Strait (~42 mol/kg DOC) will be collected, placed into ampoules, and distributed to the US and international community. Since program launch, 374,000 vials of reference waters have been distributed to 300 US and international laboratories in 45 nations, at a rate of ~27,000 vials/year in CY21. With renewal of the program for a 3-year period of support, distribution of reference materials from the upper (SSR), mid (MSR) and deep (DSR) waters of the ocean, as well as low carbon reference waters (LCW), supports the analytical work of the DOC community. Further, a new product developed during the prior award will be continued: DOC-TN Determination Kits. The kit comes with all that is necessary to analyze samples for the determination of DOC and total nitrogen (TN), including 4 calibration standards for Carbon and Nitrogen, and 4 reference materials (DSR, MSR, SSR and LCW). It also includes the Excel template, guiding the sequence of standards, CRMs and samples for each analytical run. This kit meets the needs of those who are seeking to build their analytical capabilities, following guidelines provided by the PI’s lab.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.