Collaborative Research: EAGER: Non-Lethal Tools to Estimate the Ages and Lifespans of Chondrichthyan Fishes

该项目开发了软骨鱼类老化的新方法（例如，鲨鱼、冰鞋和鳐）的DNA测序技术。在这样做的过程中，它将推进这些物种的保护，其中许多是极度濒危的，以及我们对具有高度可变寿命和生活史的分类学群体中物种衰老过程的进化理解。生物老化是生物体生命的基本组成部分。我们对衰老过程中DNA变化的理解的最新进展正在产生新的工具来探测基因组变异性，生态动态和生物体寿命之间的联系。所有脊椎动物的寿命变化，当与已知年龄的动物进行校准时，可以用来创建高度精确的“时钟”。这样的时钟有可能提供非致命的方法来确定老化的物种的保护问题，和老化的比较研究，在不同的寿命相关的物种有可能提供深入了解的机制，有助于生活史的演变。此外，该项目将为本科生和博士后学者提供培训，并通过与格鲁吉亚水族馆的联系，吸引公众和当地公众参与。生命系统通过生命史阶段循环，其特征是启动，生长和发展，繁殖，衰老和死亡。大多数关于生命史模式的进化驱动因素的假设都围绕着这样一个观点，即周期性的重置与缓慢但持续的引入新突变相结合，新突变通过重新洗牌而增强（例如，有性生殖），提供了确保在不断变化的环境中生存所必需的表型变异性。如果这是正确的，那么在快速变化的环境中的循环时间应该比在更恒定的环境中更短。该项目将开发表观遗传DNA甲基化“时钟”使用新的分子和计算方法来测量软骨鱼类的寿命，这些鱼类的寿命和生活史差异很大，并且栖息在从相对恒定的深海到温带和热带地区海面高度动态环境的环境中。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来提供支持。

This project develop new methods for aging chondrichthyans (e.g., sharks, skates, and rays) using DNA sequencing technology. In doing so, it will advance both the conservation of these species, many of which are critically endangered, and our evolutionary understanding of the aging process across species in taxonomic groups with highly variable lifespans and life histories. Biological aging is a fundamental component of organismal life. Recent advances in our understanding of changes in DNA during aging are yielding novel tools to probe the linkages between genomic variability, ecological dynamics, and organismal lifespans. These changes over the lifespan of all vertebrates, when calibrated against animals of known age, can be used to create highly accurate “clocks.” Such clocks have the potential to provide non-lethal approaches for determining aging populations of species of conservation concern, and comparative studies of aging across related species with diverse lifespans are likely to provide insight into the mechanisms contributing to life history evolution. In addition, the project will provide training for both undergraduates and a postdoctoral scholar and engage the general and local public through association with the Georgia Aquarium.Living systems cycle through life-history stages characterized by initiation, growth and development, reproduction, senescence, and death. Most hypotheses of the evolutionary drivers of life-history patterns center around the idea that periodic resetting in conjunction with the slow, but constant, introduction of new mutations augmented by reshuffling (e.g., sexual reproduction), provides the phenotypic variability necessary to ensure survival in changing environments. If this is correct, cycling times should be shorter in rapidly changing environments than they are in more constant environments. This project will develop an epigenetic DNA methylation “clock” using novel molecular and computational approaches to measure life spans in chondrichthyan fishes that vary widely in their lifespans and life histories and that inhabit environments that range from the relative constancy of the deep sea to highly dynamic environments at the sea surface in temperate and tropical regions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.