Zooplankton Energy Turnover in a Changing Environment (ZET-Change)

变化环境中的浮游动物能量周转（ZET-Change）

• 批准号: 253351799
• 负责人: Dr. Natalie Loick-Wilde
• 金额: --
• 依托单位: Leibniz-Institut für Ostseeforschung Warnemünde (IOW)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/253351799?language=en
• 关键词: Zooplankton; Energy; Turnover; Changing; Environment

Changing nitrogen and carbon biogeochemical cycles as a consequence of climate change and anthropogenic eutrophication alter marine food webs of coastal areas in an unpredictable way. These changes often manifest in the dramatic shift from nutritious algae species to lipid-poor, harmful cyanobacteria blooms that may endanger current food webs including fisheries. So far we don’t have a mechanistic understanding how lower trophic levels like zooplankton cope with such lipid-poor conditions. Zooplankton organisms play a key role in the biological productivity of the ocean as they refine algae into protein- and lipid-rich food and thus link primary to fishery production while fueling the microbial loop with their exometabolomic waste products. This ecosystem function of zooplankton may be profoundly disturbed by spreading cyanobacteria blooms because zooplankton organisms rely on a protein based metabolism only in the nutrient poor tropical ocean, while beyond the tropics they principally metabolize lipids.Previous compound-specific stable isotope work of my colleagues and me on zooplankton samples from the Baltic Sea and tropical North Atlantic proved that the understudied and generally as invariant considered amino acid pool in zooplankton rapidly changes when food concentrations are low, which challenges the current use of bulk C:N ratios as lipid: protein indicator in biogeochemical models, which we discuss in an article for the interdisciplinary journal Ecology. Our work shows that amino acids are a sensitive parameter to investigate the impact of food quality on the energy metabolism in marine food webs. In the ZET-Change Projekt this approach will be expanded to quantify the carbon and nitrogen flows through the amino acid and lipid pools of zooplankton species and communities during annual summer-time cyanobacteria blooms using the Baltic Sea as model ecosystem. We hypothesize that amino acid metabolism plays a significant role in supporting zooplankton production in the Baltic Sea, and that this process is modulated by lipid-poor conditions as found during cyanobacteria blooms and during times of low food particle concentrations. The effect on the population and ecosystem level will be tackled by exploring the exometabolomic waste product release and the flow of nitrogen and carbon into copepod eggs in combined field sampling and ship based experiments and subsequent analysis using cutting edge technologies like nanometer scale secondary ion mass spectrometry and Fourier transform ion cyclotron resonance mass spectrometry. The combination of a strong field program with a multiple method approach will place our work in a broader, basin-scale context and allow us to establish a mechanistic understanding of the role of an amino acid (a.k.a. protein) based metabolism in zooplankton during cyanobacteria blooms.

气候变化和人为富营养化导致的氮和碳生物地球化学循环的变化以不可预测的方式改变了沿海地区的海洋食物网。 这些变化往往表现在从营养丰富的藻类物种到脂质贫乏、有害的蓝藻水华的急剧转变，这可能危及包括渔业在内的当前食物网。 到目前为止，我们还没有一个机械的理解，像浮游动物这样的低营养水平如何科普这种脂质缺乏的条件。浮游动物生物在海洋的生物生产力中发挥着关键作用，因为它们将藻类提炼成富含蛋白质和脂质的食物，从而将初级生产与渔业生产联系起来，同时用它们的外代谢废物为微生物循环提供燃料。 浮游动物的这种生态系统功能可能会受到蓝藻水华蔓延的严重干扰，因为浮游动物生物只在营养贫乏的热带海洋中依赖于基于蛋白质的代谢，而在热带地区以外，它们主要代谢脂质。我和我的同事们对波罗的海和热带北大西洋浮游动物样本的特定稳定同位素工作证明，研究不足，通常被认为是不变的当食物浓度较低时，浮游动物中的氨基酸库迅速变化，这对目前使用的大量C：N比率作为生物地球化学模型中的脂质：蛋白质指标提出了挑战，我们在跨学科期刊生态学的一篇文章中对此进行了讨论。 我们的工作表明，氨基酸是一个敏感的参数来研究食物质量对海洋食物网能量代谢的影响。在ZET-变化项目中，将扩大这一方法，以波罗的海为模式生态系统，量化每年夏季蓝藻水华期间浮游动物物种和群落的氨基酸和脂质池中的碳和氮流量。 我们假设，氨基酸代谢起着重要的作用，在波罗的海浮游动物生产的支持，这一过程是调制的脂贫条件下发现蓝藻水华期间，在低食物颗粒浓度的时间。 对人口和生态系统水平的影响将通过探索exometabolomic废物释放和氮和碳流入桡足类卵结合现场采样和船基实验和随后的分析，使用尖端技术，如纳米尺度二次离子质谱和傅立叶变换离子回旋共振质谱。强有力的现场项目与多种方法的结合将使我们的工作处于更广泛的流域范围内，并使我们能够建立对氨基酸作用的机械理解。蛋白质）为基础的代谢在浮游动物在蓝藻水华。

项目成果

De novo amino acid synthesis and turnover during N2 fixation

N2 固定过程中氨基酸的从头合成和周转

• DOI: 10.1002/lno.10755
• 发表时间: 2017
• 期刊: Limnology and Oceanography
• 影响因子: 4.5
• 作者: Loick-Wilde;Eglite;Liskow;Schulz-Bull;Wasmund;Wodarg;Montoya
• 通讯作者: Montoya

Strategies of amino acid supply in mesozooplankton during cyanobacteria blooms: a stable nitrogen isotope approach

• DOI: 10.1002/ecs2.2135
• 发表时间: 2018-03-01
• 期刊: ECOSPHERE
• 影响因子: 2.7
• 作者: Eglite, Elvita;Wodarg, Dirk;Loick-Wilde, Natalie
• 通讯作者: Loick-Wilde, Natalie

The contribution of zooplankton to methane supersaturation in the oxygenated upper waters of the central Baltic Sea

• DOI: 10.1002/lno.10640
• 发表时间: 2018-01
• 期刊: Limnology and Oceanography
• 影响因子: 4.5
• 作者: O. Schmale;Janine Wäge;V. Mohrholz;N. Wasmund;U. Gräwe;G. Rehder;M. Labrenz;N. Loick‐Wilde

Microplankton biomass and diversity in the Vietnamese upwelling area during SW monsoon under normal conditions and after an ENSO event

• DOI: 10.1016/j.pocean.2017.04.007
• 发表时间: 2017-04
• 期刊: Progress in Oceanography
• 影响因子: 4.1
• 作者: N. Loick‐Wilde;Deniz Bombar;H. Doan;L. Nguyen;Anh Mai Nguyen-Thi;M. Voss;J. Dippner