Molecular Analysis of AMPA Receptor Signaling in NG2 Glial Cells

NG2 胶质细胞 AMPA 受体信号转导的分子分析

• 批准号: 254940540
• 负责人: Professor Dr. Nikolaj Klöcker
• 金额: --
• 依托单位: Institut für Neuro- und Sinnesphysiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/254940540?language=en
• 关键词: Molecular; Analysis; AMPA; Receptor; Signaling

NG2 glial cells comprise a discrete population of CNS macroglia that receives synaptic input from neurons. The physiological significance of this neuro-glial transmission remains elusive. Here, we hypothesize that fast excitatory synaptic signaling in NG2 cells, mediated by glutamate receptors of the AMPA subtype (AMPARs), serves as a critical determinant of the proliferation of NG2 cells and their putatively heterogeneous lineage progression into oligodendrocytes or other cell types. Employing an unbiased proteomic approach, we propose to analyze and functionally characterize the molecular design of AMPAR signaling in native NG2 cells with respect to their developmental stage and their localization in brain. In the next step, identified NG2 cell-specific AMPAR complex compositions, which define the trafficking and gating properties of the receptors, will be disturbed in situ to delineate their functional role in neuro-glial communication. Results are expected to be key to understand the relevance of glutamatergic signaling in NG2 cell biology.

NG2神经胶质细胞包括从神经元接收突触输入的CNS大胶质细胞的离散群体。这种神经胶质传递的生理意义仍然难以捉摸。在这里，我们假设NG2细胞中的快速兴奋性突触信号传导，由AMPA亚型的谷氨酸受体（AMPAR）介导，作为NG2细胞增殖的关键决定因素，以及它们向少突胶质细胞或其他细胞类型的分化异质谱系进展。采用公正的蛋白质组学方法，我们建议分析和功能特性的分子设计的AMPAR信号在天然NG2细胞相对于其发育阶段和它们在大脑中的定位。在下一步中，确定NG2细胞特异性AMPAR复合物组合物，其定义了受体的运输和门控特性，将被原位干扰，以描绘其在神经胶质细胞通讯中的功能作用。这些结果有望成为了解NG2细胞生物学中谷氨酸能信号相关性的关键。