Graphene/Polymer-based Fluorescent Nanozymes for Sensitive Detection of Metabolic Biotargets

用于代谢生物靶标灵敏检测的石墨烯/聚合物荧光纳米酶

• 批准号: 2304873
• 负责人: Julia Xiaojun Zhao
• 金额: $ 45万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2304873&HistoricalAwards=false
• 关键词: Graphene; Polymer; based; Fluorescent; Nanozymes

With support from the Chemical Measurement and Imaging (CMI) program in the Division of Chemistry, and co-funding from the Established Program to Stimulate Competitive Research (EPSCoR), the Chemical Structures, Dynamics, and Mechanisms – B (CSDM-B) program in the Chemistry Division, and the Biosensing Program in the Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET), Professors Julia Zhao and Diane Darland and their respective research groups at the University of North Dakota are devising a new approach to nanomaterial-based sensors, known as nanozymes, for the detection and quantitation of key metabolic biomolecules, such as glucose. They seek to create sensors usable in a range of environments and characterized by high sensitivity, easy synthesis, and good biocompatibility. In conjunction with this work, Drs. Zhao and Darland are reaching out to engage tribal high schools and colleges as well as other universities in the North Dakota University System, to encourage students from groups underrepresented in STEM (science, technology, engineering and mathematics) to consider science as a career option. Additional efforts are underway to develop hands-on advanced science lessons to help middle- and/or high-school students connect concepts from atoms to ecosystems with nanomaterials and cells. Under this award, the collaborative Zhao/Darland team at the University of North Dakota will work to develop graphene- and polymer-based fluorescent nanozymes for sensitive detection of metabolic biomolecules. The nanozymes co-catalyze targeted biomolecular reactions while providing an intense fluorescence signal proportional to the analyte concentration. Targeted attributes include high stability in various operational environments, high detection sensitivity, easy synthesis, good biocompatibility, and applicability for detection of critical cellular energetic targets. In the course of these studies, the team seeks a better understanding of the geometric, compositional, dimensional, and electrochemical characteristics of graphene- and polymer-based nanozymes. Nanozymes will be tested for toxicity and used for biotarget detection and cell imaging. Cells that display high metabolic activity and that are vulnerable to metabolic distress will be used for testing the nanozymes under normal and distressed metabolic conditions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学部化学测量和成像（CMI）计划的支持下，并由化学部的化学结构，动力学和机制- B（CSDM-B）计划，以及化学，生物工程，环境和运输系统（CBET）部门的生物传感计划共同资助，北达科他州大学的Julia Zhao教授和Diane Darland教授及其各自的研究小组正在设计一种基于纳米材料的传感器的新方法，称为纳米酶，用于检测和定量关键的代谢生物分子，如葡萄糖。他们试图创建可在一系列环境中使用的传感器，其特征在于高灵敏度，易于合成和良好的生物相容性。 在这项工作的同时，赵博士和Darland正在接触部落高中和学院以及北达科他州大学系统的其他大学，以鼓励来自STEM（科学，技术，工程和数学）代表性不足的群体的学生考虑将科学作为职业选择。正在进行更多的努力，以开发动手先进的科学课程，以帮助初中和/或高中学生连接从原子到生态系统与纳米材料和细胞的概念。根据该奖项，北达科他州大学的Zhao/Darland合作团队将致力于开发基于石墨烯和聚合物的荧光纳米酶，用于代谢生物分子的灵敏检测。纳米酶共催化靶向生物分子反应，同时提供与分析物浓度成比例的强烈荧光信号。靶向属性包括在各种操作环境中的高稳定性、高检测灵敏度、易于合成、良好的生物相容性以及用于检测关键细胞能量靶标的适用性。在这些研究过程中，该团队寻求更好地了解石墨烯和聚合物纳米酶的几何，组成，尺寸和电化学特性。纳米酶将进行毒性测试，并用于生物靶标检测和细胞成像。显示高代谢活性和易受代谢窘迫的细胞将用于在正常和窘迫代谢条件下测试纳米酶。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。