NSF PRFB FY23: Roles of gut microbiomes in herbivorous host toxin tolerance

NSF PRFB FY23：肠道微生物组在食草宿主毒素耐受性中的作用

• 批准号: 2305844
• 负责人: Bahareh Sorouri
• 金额: $ 24万
• 依托单位: Sorouri, Bahareh
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2305844&HistoricalAwards=false
• 关键词: NSF; PRFB; FY23; Roles; gut

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2023, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment, and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. Microbiomes are communities of microorganisms that are important for hosts and for the environment. This research will investigate the role of host microbiomes in digesting plants that produce toxins. Snowshoe hares and greater sage-grouse are two herbivores that eat toxic plants. They have similar digestive systems and therefore a similar environment for their gut microbiomes. Both hosts are the target of conservation efforts since their survival is threatened by pollution and climate change. Thus, understanding how herbivores overcome toxin exposure can help protect and preserve ecosystems. Furthermore, as part of the training plan, the fellow will broaden participation of diverse communities in science through teaching, science communication, mentoring, and outreach.This research will demonstrate that microbiomes perform important functions for the host as a Rule of Life. The fellow will determine the microbial genomic functions and mechanisms driving host toxin tolerance using data-driven and empirical multi-omics approaches. The research will investigate two hypotheses regarding the gut microbial role in toxin tolerance (i.e., plant secondary metabolite degradation) for snowshoe hares and greater sage-grouse: 1) the gut microbiome of snowshoe hares varies over time due to seasonal, dietary changes in toxicity, and 2) similar gut microbial taxa will drive toxin tolerance in distinct hosts that specialize on different diets. The project will incorporate metagenomic and metabolomic analyses on snowshoe hare fecal and cecal pellet collections from the Bonanza Creek Long Term Ecological Research (BNZ LTER) program sites near Fairbanks, Alaska. Furthermore, this research will classify the microbial taxa from metagenomic approaches, then will compare the microbial pathways responsible for toxin tolerance in sage-grouse and snowshoe hare cultured gut isolates with transcriptomic and metabolomic methods. Training objectives for the fellow include strengthening bioinformatic and empirical skills, expanding expertise of host-associated microbial ecology and physiology, and incorporating inclusive mentoring and teaching for historically excluded students. This project is jointly funded by Division of Biological Infrastructure in the Directorate of Biological Sciences and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动资助了2023财年NSF生物学博士后研究奖学金，即研究基因组、环境和表型之间相互作用的生命规则的综合研究。该研究金支持研究员的研究和培训，以创新的方式为生活规则领域做出贡献。微生物组是对宿主和环境都很重要的微生物群落。这项研究将调查宿主微生物组在消化产生毒素的植物中的作用。雪鞋兔和大鼠尾草松鸡是两种食草动物，吃有毒植物。它们具有相似的消化系统，因此肠道微生物组的环境相似。这两种宿主都是保护工作的目标，因为它们的生存受到污染和气候变化的威胁。因此，了解食草动物如何克服毒素暴露可以帮助保护和维护生态系统。此外，作为培训计划的一部分，该研究员将通过教学，科学交流，指导和推广来扩大不同社区对科学的参与。这项研究将证明微生物组作为生活规则对宿主发挥重要作用。该研究员将使用数据驱动和经验性多组学方法确定微生物基因组功能和驱动宿主毒素耐受性的机制。该研究将调查关于肠道微生物在毒素耐受性中的作用的两种假设（即，植物次级代谢物降解）的影响：1）雪鞋兔的肠道微生物组由于季节性、饮食毒性的变化而随时间变化，以及2）相似的肠道微生物分类群将在专门针对不同饮食的不同宿主中驱动毒素耐受性。该项目将结合宏基因组学和代谢组学分析雪鞋兔粪便和盲肠颗粒收集从博南扎溪长期生态研究（BNZ LTER）计划网站附近的费尔班克斯，阿拉斯加。此外，本研究还将从宏基因组学方法对微生物类群进行分类，然后将用转录组学和代谢组学方法比较鼠尾草松鸡和雪鞋兔培养肠道分离株中负责毒素耐受性的微生物途径。该研究员的培训目标包括加强生物信息学和经验技能，扩大宿主相关微生物生态学和生理学的专业知识，并为历史上被排斥的学生提供包容性的指导和教学。该项目由生物科学理事会生物基础设施部和刺激竞争性研究的既定计划（EPSCoR）共同资助。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。