Sustainability in Vapor-phase Nitrogen of a World-class Frozen Biorepository at the Museum of Southwestern Biology

西南生物博物馆世界级冷冻生物储存库气相氮的可持续性

• 批准号: 2308707
• 负责人: Michael Andersen
• 金额: $ 74万
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2308707&HistoricalAwards=false
• 关键词: Sustainability; Vapor; phase; Nitrogen; World

The Museum of Southwestern Biology’s (MSB) Division of Genomic Resources (DGR) is a world-class, biorepository at the University of New Mexico (UNM) for cryogenic biological materials. Founded in 1979, DGR is the largest natural history collection of frozen mammal tissues worldwide and has significant frozen tissue collections of birds, fishes, amphibians, reptiles, and parasite tissues, including critical samples from endangered species and threatened ecosystems. The DGR collection archives cryogenically preserved samples of animal tissues, whole organisms such as embryos and parasites, and purified DNA and RNA. The archive has more 700,000 frozen tissue samples from more than 300,000 vouchered specimens and is growing at 3-4 percent annually. DGR is global in scope and provides critical infrastructure for studies of genomics, climate change, molecular evolution, conservation biology, zoonotic disease, emerging pathogens, contaminants, and stable isotope ecology. Annually, about 50 loans of 2,000 specimens generate about 58 peer-reviewed publications and more than 2,800 citations. At UNM, MSB is a leader in research productivity, undergraduate and graduate student training, and public service, especially activities related to biodiversity discovery, emerging zoonotic pathogens, and science-based management of our dwindling natural resources. This project will upgrade DGR’s capacity for high-quality tissue storage in vapor-phase nitrogen. Following best practices in cryopreservation, it will substantially enhance this state-of-the-art scientific infrastructure to serve the global biodiversity science community.This project will ensure the long-term preservation, growth, and sustainability of the Division of Genomic Resources (DGR) in the Museum of Southwestern Biology (MSB). This project more than doubles DGR’s vapor-phase nitrogen storage capacity – the gold standard for frozen tissue storage – by adding six nitrogen cryotanks and a second cryohead generator to double liquid nitrogen generating capacity in the facility. This major upgrade will transfer the entire collection from tenuous ultralow freezers to secure vapor-phase nitrogen storage at -196°C. The improvements will also accommodate more than 5 years of growth, substantially reduce electrical consumption, and dramatically increase safe holding time of the frozen collection to 21 days vs. 2 hours if a freezer fails in case of catastrophic emergencies. Finally, MSB’s track record of community outreach, student training, and involvement of under-represented minorities in biodiversity science demonstrates a sincere and long-term commitment to museum-based science education. This project will recruit, support, and train a diverse population of UNM students by immersing them in best practices in museum sciences as they learn how museum collections contribute to important societal questions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

西南生物学博物馆（MSB）基因组资源（DGR）是新墨西哥大学（UNM）的世界一流的生物疗法，用于低温生物学材料。 DGR成立于1979年，是全球冷冻哺乳动物组织的最大自然历史集合，并具有大量的冷冻组织收集鸟类，鱼类，两栖动物，爬行动物和寄生虫组织，包括来自濒危物种的关键样品和威胁生态系统。 DGR收集归档了动物组织的冷冻保存样品，胚胎和寄生虫等整个生物以及纯化的DNA和RNA。该档案馆有来自300,000多个代金券标本的700,000个冷冻组织样品，每年增长3-4％。 DGR在范围内是全球性的，为基因组学，气候变化，分子进化，保护生物学，人畜共患病，新兴病原体，污染物和稳定的同位素生态的研究提供了关键的基础设施。每年，约有50个贷款，共有2,000个标本，产生约58个同行评审的出版物和超过2,800个引用。在UNM中，MSB是研究生产力，本科生和研究生培训以及公共服务的领导者，尤其是与生物多样性发现，新兴的人畜共患病原体以及基于科学的自然资源管理有关的活动。该项目将升级DGR在蒸汽氮中高质量组织存储的能力。遵循冷冻保存方面的最佳实践，它将大大提高为全球生物多样性科学界服务的最先进的科学基础设施。该项目将确保西南生物学博物馆（MSB）的基因组资源分工（DGR）的长期准备，增长和可持续性。该项目通过添加六个氮气冷冻机和第二个冷冻发电机来使DGR的蒸气相氮储能（用于冷冻组织存储的金标准）翻倍，以使设施中的液氮产生能力加倍。这次重大升级将使整个集合从脆弱的超冰柜转移到-196°C的蒸汽相氮。这些改进还将适应超过5年的增长，大大降低电消耗，并大大增加冷冻收集的安全持有时间，而如果在发生灾难性的紧急情况下冰柜失败，则冻结的收集时间与2小时相比2小时。最后，MSB在社区外展，学生培训以及代表性不足的少数群体参与生物多样性科学方面的记录表明了对基于博物馆的科学教育的真诚和长期承诺。该项目将通过将博物馆收藏如何促进重要社会问题的最佳实践中的最佳实践来招募，支持和培训UNM学生的多样性人群。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子优点和更广泛影响的审查标准来通过评估而被视为珍贵的支持。