NSF-BSF: IIBR Instrumentation: Photonic Band Gap Resonators for High-Field Dynamic Nuclear Polarization of Biological Macromolecules

NSF-BSF：IIBR 仪器：用于生物大分子高场动态核极化的光子带隙谐振器

• 批准号: 2311042
• 负责人: Alexander Nevzorov
• 金额: $ 99.84万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2027-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2311042&HistoricalAwards=false
• 关键词: NSF; BSF; IIBR; Instrumentation; Photonic

An award is made to North Carolina State University (NCSU, USA) with support from the Infrastructure Innovation Program for Biological Research in the Division of Biological Infrastructure and the Chemical Measurement and Imaging Program in the Division of Chemistry to considerably – by up to several orders of magnitude – improve sensitivity of Nuclear Magnetic Resonance (NMR) spectroscopy by employing pulsed high-frequency methods of Dynamic Nuclear Polarization (DNP). This first-of-its kind spectrometer will be developed in partnership with Tel Aviv University (TAU, Israel), thereby strengthening scientific collaboration between the two Nations. Such gains in sensitivity will expand the applicability of NMR methods to some of the most challenging problems of structural biology and, potentially, make the method suitable for studying protein structure and function in living cells. This highly interdisciplinary collaborative project will provide unique training opportunities for students with backgrounds in biology, physical chemistry, spin physics, and millimeter-wave technologies.The project is aimed at transforming DNP NMR methods by significantly expanding the photonic band-gap resonator technology invented at NCSU from the current 200 GHz electron resonance frequency to 400 GHz and take advantage of the expertise of the TAU team in pulse shaping and cryoprobe development. The instrument will operate at 400 GHz electron and 600 MHz proton NMR frequencies, which will be highly beneficial for higher resolution and sensitivity. Coherent manipulation of the electronic spin states will be achieved by combining state-of-the- art digital technologies and recent advances in solid-state millimeter-wave devices. The spectrometer will serve as a unique platform for developing new methods for transferring spin polarization in DNP. The method is expected to yield novel structural and dynamic information on biological macromolecules as compared to conventional NMR spectroscopy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

北卡罗来纳州州立大学（NCSU，USA）在生物基础设施部门的生物研究基础设施创新计划和化学部门的化学测量和成像计划的支持下，通过采用脉冲高分辨率技术，大大提高了核磁共振（NMR）光谱的灵敏度，动态核极化（DNP）的频率方法。这是第一台光谱仪，将与以色列特拉维夫大学（TAU）合作开发，从而加强两国之间的科学合作。这种灵敏度的提高将扩大NMR方法对结构生物学中一些最具挑战性的问题的适用性，并可能使该方法适用于研究活细胞中的蛋白质结构和功能。这个高度跨学科的合作项目将为具有生物学，物理化学，自旋物理，和毫米波技术。该项目旨在通过显着扩展光子带来改变DNP NMR方法-NCSU发明的间隙谐振器技术，从目前的200 GHz电子谐振频率到400 GHz，并利用TAU的专业知识脉冲整形和冷冻探针开发团队。该仪器将在400 GHz电子和600 MHz质子NMR频率下工作，这将非常有利于提高分辨率和灵敏度。电子自旋态的相干操纵将通过结合最先进的数字技术和固态毫米波器件的最新进展来实现。该光谱仪将作为一个独特的平台，用于开发在DNP中转移自旋极化的新方法。 与传统的核磁共振光谱相比，该方法有望获得生物大分子的新结构和动态信息。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。