Identification of tumor affine peptides in head and neck tumors by display techniques

通过显示技术鉴定头颈肿瘤中的肿瘤亲和肽

• 批准号: 256151267
• 负责人: Professor Dr. Uwe Haberkorn
• 金额: --
• 依托单位: Neurochirurgische Klinik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/256151267?language=en
• 关键词: Identification; tumor; affine; peptides; head

This proposal aims at identifying tumor affine peptides against head and neck tumors using high throughput techniques. These molecules will be used for imaging after labeling with radioisotopes or fluorescence dyes or for therapy after labeling with alpha or beta emitting isotopes or coupling to chemotherapeutic drugs. The identification of new ligands with affinity towards head and neck cancer is done with peptide libraries and based on phage or ribosome display and differentially expressed parts of tumor cell membranes. These tumor cell membranes will be isolated using a new proteomics technology. This proteomics technology will also be used at later stages for the identification of the target structures for the binding peptides. In case of newly identified target structures for head and neck cancer a characterization of their expression in a larger population of head and neck tumors is planned. The preclinical evaluation of the ligands is performed in binding studies in cell lines, tissues slices, biodistribution and imaging experiments in tumor bearing mice.

该建议旨在使用高通量技术鉴定针对头颈部肿瘤的肿瘤亲和肽。这些分子将在用放射性同位素或荧光染料标记后用于成像，或在用α或β发射同位素标记后用于治疗或与化疗药物偶联。对头颈癌具有亲和力的新配体的鉴定是利用肽文库并基于噬菌体或核糖体展示和肿瘤细胞膜的差异表达部分来完成的。这些肿瘤细胞膜将使用新的蛋白质组学技术分离。这种蛋白质组学技术也将在后期阶段用于鉴定结合肽的靶结构。在新鉴定的头颈癌靶结构的情况下，计划在更大的头颈肿瘤群体中表征其表达。配体的临床前评价在细胞系、组织切片中的结合研究、荷瘤小鼠中的生物分布和成像实验中进行。

项目成果

Identification of a Novel ITGαvβ6-Binding Peptide Using Protein Separation and Phage Display

• DOI: 10.1158/1078-0432.ccr-16-3217
• 发表时间: 2017-08-01
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Altmann, Annette;Sauter, Max;Haberkorn, Uwe
• 通讯作者: Haberkorn, Uwe