BRITE Fellow: Rigid Engineered Living Materials

BRITE 研究员：刚性工程活性材料

• 批准号: 2342239
• 负责人: Christopher Hernandez
• 金额: $ 96.28万
• 依托单位: University of California-San Francisco
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-15 至 2027-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2342239&HistoricalAwards=false
• 关键词: BRITE; Fellow; Rigid; Engineered; Living

This Boosting Research Ideas for Transformative and Equitable Advances in Engineering (BRITE) Fellow grant will establish basic scientific and design approaches needed to combine materials used in engineering applications (plastics, metals, ceramics, etc.) with living organisms (bacteria). The addition of living cells to engineering materials has the potential to instill biological traits (growth, healing, etc.) to the materials used to make vehicles, buildings, and commercial products. In particular, the ability of living cells to continuously repair a material has the potential to reduce financial and environmental costs generated when worn parts/devices are repaired or replaced. The creation of new longer-lasting materials has the potential to reduce energy needs associated with material manufacturing which are responsible for 25 percent of global carbon emissions. In addition to advancing engineering science, the work includes efforts to maintain US leadership in technology by increasing domestic engineering talent pools through broadening participation by members of underrepresented groups. Specifically, the efforts involve building a network of engineering faculty to coordinate volunteer service, mentorship and advocacy toward the long-term goal of achieving demographic parity in engineering by the US Hispanic population.Most convergent research spanning engineering and biology focuses on the use of engineering principals to address challenges in biology. In contrast, this BRITE Fellow project focuses on the transformative concept of using biological systems to address challenges in engineering. This project seeks to advance the field of Engineered Living Materials by focusing on the design and function of rigid, load carrying materials functionalized by the presence of living organisms. The project addresses the following research questions: (i) What design principles are required to maintain viability of resident cells within rigid engineered materials? (ii) What are the best ways to populate or repopulate a rigid material using living cells? (iii) What existing manufacturing techniques can be used to create materials easily populated by bacteria? and (iv) How could mechanically sensitive bacteria be used within the materials to sense mechanical damage? The research uses a naturally occurring rigid living material, bone, as a biological inspiration. Nanofluidic devices are used to identify functional design principles for channel morphology, nutrient delivery, and the utility of externally applied fluid pressure and/or mechanical loading. The findings will be applicable across several classes of rigid materials (polymers, metals, ceramics) and several types of cells researched for use in engineered living materials (bacteria, fungi, microalgae, etc.).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个促进工程（BRITE）研究员赠款的变革和公平进步的研究思路将建立所需的基本科学和设计方法，以结合工程应用（塑料，金属，陶瓷等）中使用的联合收割机材料。生物体（细菌）。在工程材料中添加活细胞有可能灌输生物特性（生长，愈合等）。到用于制造车辆、建筑物和商业产品的材料。特别是，活细胞持续修复材料的能力有可能减少修复或更换磨损部件/设备时产生的财务和环境成本。新材料的创造有可能减少与材料制造相关的能源需求，而材料制造占全球碳排放量的25%。除了推进工程科学，这项工作还包括通过扩大代表性不足群体的成员参与来增加国内工程人才库，从而努力保持美国在技术方面的领导地位。具体而言，这些努力涉及建立一个工程教师网络，以协调志愿服务，指导和宣传，以实现美国西班牙裔人口在工程学方面的人口平等的长期目标。大多数跨越工程学和生物学的融合研究都集中在使用工程学原理来解决生物学方面的挑战。相比之下，这个BRITE研究员项目的重点是利用生物系统来应对工程挑战的变革性概念。该项目旨在通过专注于由生物体功能化的刚性承载材料的设计和功能来推进工程生物材料领域。该项目解决了以下研究问题：（一）需要什么样的设计原则，以保持刚性工程材料内的常驻细胞的活力？(ii)使用活细胞填充或重新填充刚性材料的最佳方法是什么？(iii)现有的哪些制造技术可以用来制造容易被细菌繁殖的材料？以及（iv）如何在材料中使用机械敏感细菌来感知机械损伤？这项研究使用了一种自然存在的刚性生物材料，骨头，作为生物灵感。纳米流体装置用于确定通道形态、营养物递送以及外部施加的流体压力和/或机械负载的效用的功能设计原理。这些发现将适用于几类刚性材料（聚合物，金属，陶瓷）和几种类型的细胞，用于工程生物材料（细菌，真菌，微藻等）。该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估的支持。

项目成果

Solute Transport in Engineered Living Materials Using Bone‐Inspired Microscale Channel Networks

使用骨启发的微尺度通道网络进行工程活性材料中的溶质运输

• DOI: 10.1002/adem.202301032
• 发表时间: 2023
• 期刊: Advanced Engineering Materials
• 影响因子: 3.6
• 作者: van Wijngaarden, Ellen W.;Bratcher, Samantha;Lewis, Karl J.;Hernandez, Christopher J.
• 通讯作者: Hernandez, Christopher J.