Collaborative Research: MTM1: Microbial Genomic, Transcriptomic, and Survival Response to Common Built Environment Lighting

合作研究：MTM1：微生物基因组、转录组和对常见建筑环境照明的生存反应

• 批准号: 2401220
• 负责人: Kevin Van Den Wymelenberg
• 金额: $ 32.5万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2401220&HistoricalAwards=false
• 关键词: Collaborative; Research; MTM1; Microbial; Genomic

The vast majority of individuals in the world spend at least 90% of their time indoors. While indoors, these individuals are exposed to a combination of artificial and natural light extending into the nighttime hours. In addition to our own light exposure, indoor microorganisms such as bacteria and fungi, are also exposed to differing combinations of natural and artificial light. At present, the consequences of light exposure on the microbial world are poorly understood. The goal of this research is to examine the consequences of varying light exposure on the microbial world. By creating a better understanding of the impact building illumination has on microorganisms, researchers can better understand how it might be possible to control their activity, survival, and functions within the built environment. Additional benefits to society resulting from this research result from science experiences for K-12 teachers to teach them how science is performed through experiential learning. This effort will enhance STEM education through incorporation of teacher’s science training in the classroom. Further benefits result disseminating results through public talks and social media outreach focused on children and young adults.The goal of this research project is to understand the mechanisms underlying microbial responses to architectural lighting conditions within the built environment. This will be achieved by assessing the impact of light on microbial communities using viability quantitative polymerase chain reaction, high resolution genome sequencing, and transcriptomic analysis. Axenic cultures isolated in microcosms will be exposed to conditions simulating typical indoor lighting, reflectance, spatial, temperature, and humidity conditions in the built environment. Varying light exposure include presence/absence of light, as well as diurnal light cycles typical in office environments. Following characterization of the axenic cultures, mock microbial communities composed of the axenic cultures will be exposed to the same lighting conditions, permitting discernment of light-associated impacts in survival and transcriptional output from those communities induced through competition. High-resolution dynamics of microbial survival and transcriptional expression will be assessed from individual species function to reveal information on the mechanisms of symbiosis, survival, and demise of microbial component species and communities associated with light in the built environment.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

世界上绝大多数人在室内至少花费90％的时间。在室内时，这些人接触到夜间延伸到夜间的人造和自然光的组合。除了我们自己的光线曝光外，诸如细菌和真菌之类的室内微生物还暴露于自然和人造光的不同组合。目前，光暴露对微生物世界的后果知之甚少。这项研究的目的是检查微生物世界的光照暴露的后果。通过更好地了解建筑物照明对微生物的影响，研究人员可以更好地了解如何控制其活动，生存和功能。由K-12老师的科学经验导致社会带来的额外好处，以教他们如何通过经验学习进行科学。这项工作将通过在课堂上的教师科学培训来增强STEM教育。进一步的好处结果通过公众对话和社交媒体外展传播结果，这些媒体关注儿童和年轻人。该研究项目的目的是了解建筑环境中对建筑照明条件的微生物反应的基础机制。这将通过评估光对微生物中分离的微生物轴承培养物的影响来实现，这将暴露于模拟建筑环境中典型的室内照明，反射率，空间，温度和湿度条件的条件。不同的光线暴露包括在办公环境中典型的光周期以及昼夜光周期。在表征轴承培养物的表征之后，由轴承培养物组成的模拟微生物群落将暴露于相同的照明条件下，从而辨别着通过竞争引起的这些群落的生存和转录输出中的光相关影响。微生物存活和转录表达的高分辨率动态将从单个物种功能进行评估，以揭示有关共生，生存和与光中环境中与光相关的微生物成分物种和社区消亡机制的信息。该奖项反映了NSF的法定任务，并通过评估基础的智力效果和广泛的范围来评估，并被视为诚实。