Collaborative Research: MTM1: Microbial Genomic, Transcriptomic, and Survival Response to Common Built Environment Lighting

合作研究：MTM1：微生物基因组、转录组和对常见建筑环境照明的生存反应

• 批准号: 2025483
• 负责人: Kevin Van Den Wymelenberg
• 金额: $ 32.5万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-11-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2025483&HistoricalAwards=false
• 关键词: Collaborative; Research; MTM1; Microbial; Genomic

The vast majority of individuals in the world spend at least 90% of their time indoors. While indoors, these individuals are exposed to a combination of artificial and natural light extending into the nighttime hours. In addition to our own light exposure, indoor microorganisms such as bacteria and fungi, are also exposed to differing combinations of natural and artificial light. At present, the consequences of light exposure on the microbial world are poorly understood. The goal of this research is to examine the consequences of varying light exposure on the microbial world. By creating a better understanding of the impact building illumination has on microorganisms, researchers can better understand how it might be possible to control their activity, survival, and functions within the built environment. Additional benefits to society resulting from this research result from science experiences for K-12 teachers to teach them how science is performed through experiential learning. This effort will enhance STEM education through incorporation of teacher’s science training in the classroom. Further benefits result disseminating results through public talks and social media outreach focused on children and young adults.The goal of this research project is to understand the mechanisms underlying microbial responses to architectural lighting conditions within the built environment. This will be achieved by assessing the impact of light on microbial communities using viability quantitative polymerase chain reaction, high resolution genome sequencing, and transcriptomic analysis. Axenic cultures isolated in microcosms will be exposed to conditions simulating typical indoor lighting, reflectance, spatial, temperature, and humidity conditions in the built environment. Varying light exposure include presence/absence of light, as well as diurnal light cycles typical in office environments. Following characterization of the axenic cultures, mock microbial communities composed of the axenic cultures will be exposed to the same lighting conditions, permitting discernment of light-associated impacts in survival and transcriptional output from those communities induced through competition. High-resolution dynamics of microbial survival and transcriptional expression will be assessed from individual species function to reveal information on the mechanisms of symbiosis, survival, and demise of microbial component species and communities associated with light in the built environment.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

世界上绝大多数人至少有90%的时间在室内度过。在室内，这些人暴露在人造光和自然光的结合下，一直持续到夜间。除了我们自己的光暴露，室内微生物，如细菌和真菌，也暴露在不同的自然光和人造光的组合。目前，人们对光照对微生物世界的影响知之甚少。这项研究的目的是研究不同光照对微生物世界的影响。通过更好地了解建筑照明对微生物的影响，研究人员可以更好地了解如何控制它们在建筑环境中的活动，生存和功能。这项研究给社会带来的额外好处是，K-12教师的科学经验教会他们如何通过体验式学习进行科学。这一努力将通过将教师的科学培训纳入课堂来加强STEM教育。进一步的好处是通过公众演讲和社交媒体宣传儿童和年轻人为重点传播结果。本研究项目的目标是了解建筑环境中微生物对建筑照明条件的反应机制。这将通过使用活力定量聚合酶链反应、高分辨率基因组测序和转录组学分析评估光对微生物群落的影响来实现。将在微观世界中分离的无菌培养物暴露于模拟建筑环境中典型室内照明、反射率、空间、温度和湿度条件的条件下。不同的光暴露包括光的存在/不存在，以及办公室环境中典型的昼夜光周期。在无菌培养物的表征之后，将由无菌培养物组成的模拟微生物群落暴露于相同的光照条件，从而允许辨别通过竞争诱导的来自那些群落的存活和转录输出中的光相关影响。高分辨率动态微生物生存和转录表达将从单个物种的功能进行评估，以揭示与建筑环境中的光相关的微生物组分物种和群落的共生，生存和消亡机制的信息。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。