Regulatory role of non-coding RNAs and their homeostasis by autophagy in the progression of atherosclerosis (B05)

非编码 RNA 的调节作用及其通过自噬实现的稳态在动脉粥样硬化进展中的作用 (B05)

• 批准号: 260030156
• 金额: --
• 依托单位: Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/260030156?language=en
• 关键词: Regulatory; role; non; coding; RNAs

The crosstalk between autophagy and non-coding RNAs in atherosclerosis remains unclear. Bulk, single-cell, and spatial RNA sequencing of human atherosclerosis identified several deregulated ncRNAs with potential relevance in autophagy (Aim 1). Among them, HAND2-AS1 interacts with autophagy-related mediators. Its functional role in vascular disease progression will be investigated (Aim 2). Further, we will study autophagy-induced ncRNA regulation through advanced proteomic and transcriptomic approaches (Aim 3). The contribution to atherosclerosis of identified RNA-binding proteins (e.g., MEX3A) will finally be studied in transgenic mice models (Aim 4).

动脉粥样硬化中自噬和非编码RNA之间的串扰仍不清楚。人类动脉粥样硬化的整体、单细胞和空间RNA测序鉴定了几种在自噬中具有潜在相关性的失调ncRNA（目的1）。其中，HAND 2-AS 1与自噬相关介质相互作用。将研究其在血管疾病进展中的功能作用（目的2）。此外，我们将通过先进的蛋白质组学和转录组学方法研究自噬诱导的ncRNA调控（目的3）。已鉴定的RNA结合蛋白（例如，MEX 3A）将最终在转基因小鼠模型中进行研究（目的4）。