Höchstauflösendes Fluoreszenzmikroskop

高分辨率荧光显微镜

• 批准号: 260348792
• 金额: --
• 依托单位: Klinikum der Universität München
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/260348792?language=en
• 关键词: chstaufl; sendes; Fluoreszenzmikroskop

To identify new strategies for the treatment of disease, advanced imaging strategies with nanoscale resolution are essential to reveal intra- and extracellular processes and the morphology of processes of healthy and diseased cells and tissues in a physiological environment. The currently available optical microscopic techniques and other imaging modalities for basic science are either not applicable to study viable samples or do not offer the resolution for gaining access to the intracellular environment (< 100 nm) of disease processes. We therefore aim to obtain and employ the state-of-the-art "three dimensional stimulated emission depletion" (STED X3) microscopy. STED will enable us to acquire ultra-detailed information on structural and functional processes relevant to disease, in particular cardiovascular pathogenesis. The applicability of STED in live cells or tissues combined with its nanoscale resolution will unravel new mechanisms and hitherto invisible structures in a pathogenic context. This will contribute to a better understanding and treatment of disease.

为了确定疾病治疗的新策略，具有纳米分辨率的先进成像技术对于揭示生理环境中健康和患病细胞和组织的细胞内和细胞外过程以及过程的形态至关重要。目前可用的光学显微镜技术和其他基础科学成像方式要么不适用于研究活的样本，要么不能提供进入疾病过程的细胞内环境(&lt；100 nm)的分辨率。因此，我们的目标是获得并使用最先进的“三维受激辐射耗尽”(STED X3)显微镜。STED将使我们能够获得与疾病相关的结构和功能过程的超详细信息，特别是心血管发病机制。STED在活细胞或组织中的适用性与其纳米级分辨率相结合，将在致病背景下揭开新的机制和迄今看不见的结构。这将有助于更好地了解和治疗疾病。

项目成果

Dimeric Glycoprotein VI Binds to Collagen but Not to Fibrin

• DOI: 10.1160/th17-04-0302
• 发表时间: 2018-02-01
• 期刊: THROMBOSIS AND HAEMOSTASIS
• 影响因子: 6.7
• 作者: Ebrahim, Mariam;Jamasbi, Janina;Siess, Wolfgang
• 通讯作者: Siess, Wolfgang

CD27 co-stimulation increases the abundance of regulatory T cells and reduces atherosclerosis in hyperlipidaemic mice

CD27 共刺激可增加高脂血症小鼠调节性 T 细胞的丰度并减少动脉粥样硬化

• DOI: 10.1093/eurheartj/ehx517
• 发表时间: 2017
• 期刊: European Heart Journal
• 影响因子: 39.3
• 作者: Winkels H;Meiler S;Lievens D;Engel D;Spitz C;Bürger C;Beckers L;Dandl A;Reim S;Ahmadsei M;Hartwig H;Holdt LM;Hristov M;Megens RTA;Schmitt MM;Biessen EA;Borst J;Faussner A;Weber C;Lutgens E;Gerdes N
• 通讯作者: Gerdes N

Optical imaging innovations for atherosclerosis research: multiphoton microscopy and optical nanoscopy.

动脉粥样硬化研究的光学成像创新：多光子显微镜和光学纳米显微镜

• DOI: 10.1161/atvbaha.115.304875
• 发表时间: 2015
• 期刊: Arteriosclerosis, Thrombosis, & Vascular Biology
• 作者: Megens RTA;Bianchini M;Schmitt MNN;Weber C
• 通讯作者: Weber C

Chronic Intake of the Selective Serotonin Reuptake Inhibitor Fluoxetine Enhances Atherosclerosis

长期摄入选择性血清素再摄取抑制剂氟西汀会加剧动脉粥样硬化

• DOI: 10.1161/atvbaha.117.310536
• 发表时间: 2018
• 期刊: Arteriosclerosis, Thrombosis, and Vascular Biology
• 作者: Guillamat-Prats R;Rinne P;Ring L;Söhnlein O;Blanchet X;Megens;Döring Y;Weber C;Faussner A;Steffens S
• 通讯作者: Steffens S

CD70 limits atherosclerosis and promotes macrophage function

• DOI: 10.1160/th16-04-0318
• 发表时间: 2016-10
• 期刊: Thrombosis and Haemostasis
• 影响因子: 6.7
• 作者: H. Winkels;Svenja Meiler;E. Smeets;D. Lievens;D. Engel;Charlotte Spitz;C. Bürger;P. Rinne;Linda Beckers;Angelika Dandl;S. Reim;M. Ahmadsei;J. van den Bossche;L. Holdt;R. Megens;Martin M. N. Schmitt;M. D. de Winther;E. Biessen;J. Borst;A. Faussner;C. Weber;E. Lutgens;N. Gerdes