Höchstauflösendes Fluoreszenzmikroskop

为了确定治疗疾病的新策略，具有纳米级分辨率的先进成像策略对于揭示生理环境中健康和患病细胞和组织的细胞内和细胞外过程以及过程的形态是必不可少的。目前可用的光学显微镜技术和基础科学的其他成像模式要么不适用于研究可行的样品，要么不提供获得疾病过程的细胞内环境（< 100 nm）的分辨率。因此，我们的目标是获得和采用最先进的“三维受激发射耗尽”（STED X3）显微镜。STED将使我们能够获得与疾病相关的结构和功能过程的超详细信息，特别是心血管发病机制。STED在活细胞或组织中的适用性及其纳米级分辨率将揭示致病背景下的新机制和迄今为止不可见的结构。这将有助于更好地了解和治疗疾病。

To identify new strategies for the treatment of disease, advanced imaging strategies with nanoscale resolution are essential to reveal intra- and extracellular processes and the morphology of processes of healthy and diseased cells and tissues in a physiological environment. The currently available optical microscopic techniques and other imaging modalities for basic science are either not applicable to study viable samples or do not offer the resolution for gaining access to the intracellular environment (< 100 nm) of disease processes. We therefore aim to obtain and employ the state-of-the-art "three dimensional stimulated emission depletion" (STED X3) microscopy. STED will enable us to acquire ultra-detailed information on structural and functional processes relevant to disease, in particular cardiovascular pathogenesis. The applicability of STED in live cells or tissues combined with its nanoscale resolution will unravel new mechanisms and hitherto invisible structures in a pathogenic context. This will contribute to a better understanding and treatment of disease.