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An Effector- and Genomics-Assisted Pipeline for Necrotrophic Pathogen Resistance Breeding in Wheat

小麦坏死营养型病原体抗性育种的效应器和基因组学辅助管道

基本信息

批准号：
263641700
负责人：
Dr. James Cockram
金额：
--
依托单位：
Institut für Pflanzenbau und Pflanzenzüchtung
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/263641700?language=en
关键词：
Effector Genomics Assisted Pipeline Necrotrophic

项目摘要

EfectaWheat is a partnership of five complementary institutes from the UK (NIAB), Germany (LFL), Denmark (AARHUS), Norway (NMBU) and Australia (CCDM, self-funded), as well as a subcontract to the UK governmental department, FERA. The partners form a multidisciplinary team focusing on the economically important wheat leaf spot group (LSG) of necrotrophic pathogens: Parastagonospora nodorum (Pn, cause of Septoria nodorum blotch; SNB), Zymoseptoria tritici (Zt, Septoria tritici blotch; STB) and Pyrenophora tritici-repentis (Ptr, tan spot; TS). High-resolution wheat genetic mapping populations (available at NIAB and LFL) and high-density genotyping will be combined with effector-based tools, pathogen re-sequencing approaches (CCDM), and expertise in pathogen diagnostics, virulence assessment (FERA, LFL, AARHUS), and field pathotesting across all partner countries (NMBU, NIAB, AARHUS, LFL). These combine to deliver a genomics- and effector-based pipeline for the genetic dissection of LSG host-pathogen interactions in Europe. The unique positions of partners at the interface between crop research and translation ensure effective dissemination of project outputs to European agri-industry. This approach has been successfully implemented by CCDM for SNB and TS in Australia, where it is forecasted to reduce losses by ~EUR100m per annum by 2020. This project will extend the approach to Europe. Specifically, it will:(1) Establish relative impact of LSG pathogens in target EU countries using molecular approaches.(2) Identify new TS and SNB effectors.(3) Determine the effector profile of Pn and Ptr isolates and the sensitivity of cultivars to effectors. (4) Screen TS, SNB and STB effectors against high-resolution wheat germplasm resources. (5) Resolve multiple LSG effector and field resistance QTLs at high-resolution.(6) Establish prevention and management strategies against multiple LSG pathogens. (7) Deliver tools (effectors and markers) for LSG resistance breeding to EU breeders and researchers.All resources will be made available to the research and industrial communities to facilitate practical exploitation. To help ensure effective uptake of information and data, dissemination activities will focus principally around:(1) Existing dissemination infrastructures and materials provided by project partners, aimed at private breeding companies, agronomists and growers.(2) Existing bi-lateral relationships between partner institutes and wheat breeding companies.(3) On-farm: demonstration and presentation at NIAB Innovation Farm.(4) On-line: via the project web-site, to include all data, or links to data, as it emerges.

EfectaWheat 是英国 (NIAB)、德国 (LFL)、丹麦 (AARHUS)、挪威 (NMBU) 和澳大利亚（CCDM，自筹资金）五个互补机构的合作伙伴，也是英国政府部门 FERA 的分包合同。合作伙伴组成了一个多学科团队，专注于经济上重要的坏死营养病原体小麦叶斑病群 (LSG)：Parastagonospora nodorum（Pn，导致斑针孢菌斑病的原因；SNB）、小麦发酵病斑菌（Zt，小麦壳针孢斑病；STB）和小麦白斑病菌（Ptr，tan）现货； TS）。高分辨率小麦遗传图谱群体（NIAB 和 LFL 提供）和高密度基因分型将与基于效应子的工具、病原体重测序方法 (CCDM) 以及病原体诊断、毒力评估（FERA、LFL、AARHUS）和所有伙伴国家（NMBU、NIAB、AARHUS、LFL）的现场病理测试方面的专业知识相结合。这些结合起来，为欧洲 LSG 宿主-病原体相互作用的基因解析提供了基于基因组学和效应子的管道。合作伙伴在作物研究和转化之间的独特地位确保了项目成果向欧洲农业工业的有效传播。 CCDM 已在澳大利亚为 SNB 和 TS 成功实施了这一方法，预计到 2020 年每年可减少约 1 亿欧元的损失。该项目将把该方法推广到欧洲。具体来说，它将：(1) 使用分子方法确定 LSG 病原体在目标欧盟国家的相对影响。(2) 识别新的 TS 和 SNB 效应子。(3) 确定 Pn 和 Ptr 分离株的效应子特征以及品种对效应子的敏感性。 (4)针对高分辨率小麦种质资源筛选TS、SNB和STB效应子。 (5) 高分辨率解析多个LSG效应子和田间抗性QTL。(6) 建立针对多种LSG病原体的预防和管理策略。 (7) 向欧盟育种者和研究人员提供用于LSG抗性育种的工具（效应器和标记）。所有资源将提供给研究和工业界，以促进实际开发。为了帮助确保信息和数据的有效利用，传播活动将主要侧重于：(1) 项目合作伙伴提供的现有传播基础设施和材料，针对私营育种公司、农艺师和种植者。(2) 合作机构和小麦育种公司之间现有的双边关系。(3) 农场：在 NIAB 创新农场进行演示和介绍。(4) 在线：通过项目网站，包括所有数据，或出现的数据链接。