Function of thylakoid low molecular weight proteins and assembly factors in the biogenesis, activity and stability of photosynthetic complexes

类囊体低分子量蛋白和组装因子在光合复合物的生物发生、活性和稳定性中的作用

• 批准号: 263978931
• 负责人: Privatdozent Dr. Jörg Meurer
• 金额: --
• 依托单位: Lehrstuhl für Molekularbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/263978931?language=en
• 关键词: Function; thylakoid; low; molecular; weight

An intriguing feature of the photosynthetic complexes of the thylakoid membrane is their high number of low molecular weight (LMW) proteins, which are nuclear and plastid encoded. Here we investigate the distinct function of assembly factors and of individual LMW proteins in the biogenesis, light absorbance, electron transport processes, phosphorylation, stabilisation and repair mainly of photosystem (PS)II using available knockouts of corresponding nuclear and plastid genes in Arabidopsis and tobacco, respectively. Furthermore, the mutant repertoire will be used to identify the composition of subcomplexes appearing during assembly and their localisation within the thylakoid system using native gels, co-immunoprecipitation (Co-IP), chromatography, cross-linking, tagged proteins, fractionation techniques and mass spectrometry. We provide clear evidence that the LMW membrane protein PsbN, which hitherto was annotated as PSII subunit, is not part of this complex but essentially required for assembly of its heterodimeric reaction centre. Direct interaction partners of PsbN and proteins associated in several precomplexes together with PsbN will be identified, already obtained interacting factors will be verified and analysed using split-ubiquitin assays, protein overlay assays, SPR spectrometry, Co-IP with tagged versions of PsbN and mass spectrometry. The addressed issues assist in our understanding of the stepwise assembly, light capture, transfer of electrons within and outgoing of PSII, water splitting and O2 evolution, membrane dynamics and recovery of the susceptible PSII from photoinhibition and heat inactivation.

类囊体膜的光合复合物的一个有趣特征是它们的低分子量（LMW）蛋白数量的大量，这些蛋白是核和质体编码的。在这里，我们研究了组装因子和单个LMW蛋白在生物发生，光吸光度，电子传输过程，磷酸化过程，稳定和修复（PS）II（PS）II中的独特功能，分别使用拟南芥和烟草中的相应核和质体基因的可用敲除。此外，使用天然凝胶，共免疫沉淀（CO-IP），色谱，交联，标记的蛋白质，分数技术和质量图和质谱法，使用突变曲目来识别组装过程中出现的亚复合物及其在类囊体系统中出现的亚复合物的组成。我们提供明确的证据表明，LMW膜蛋白PSBN迄今被注释为PSII亚基，不是该复合物的一部分，而是其异二聚体反应中心的组装所必需的。将确定与PSBN的直接相互作用伙伴和与PSBN相关的蛋白质的直接相互作用伙伴，将使用分裂泛素分析，蛋白质覆盖测定，SPR光谱法，co-IP与PSBN和质谱法的标签版本进行验证和分析。解决的问题有助于我们理解逐步组装，光捕获，PSII内外的电子传递，水分分割和O2进化，膜动力学以及易感PSII从光抑制和热量失活中恢复。

项目成果

The Low Molecular Weight Protein PsaI Stabilizes the Light-Harvesting Complex II Docking Site of Photosystem I1

低分子量蛋白 PsaI 稳定光系统 I1 的光捕获复合物 II 对接位点

• DOI: 10.1104/pp.16.00647
• 发表时间: 2016
• 期刊: Plant Physiology
• 影响因子: 7.4
• 作者: Plöchinger M;Torabi S;Rantala M;Tikkanen M;Suorsa M;Jensen PE;Aro EM;Meurer J
• 通讯作者: Meurer J

The Arabidopsis Tellurite resistance C protein together with ALB3 is involved in photosystem II protein synthesis.

拟南芥亚碲酸盐抗性 C 蛋白与 ALB3 一起参与光系统 II 蛋白合成

• DOI: 10.1111/tpj.12474
• 发表时间: 2014
• 期刊: The Plant journal : for cell and molecular biology
• 作者: Schneider A;Steinberger I;Strissel H;Kunz HH;Manavski N;Meurer J;Burkhard G;Jarzombski S;Schünemann D;Geimer S;Flügge UI;Leister D
• 通讯作者: Leister D

Localization and Topology of Thylakoid Membrane Proteins in Land Plants

陆地植物类囊体膜蛋白的定位和拓扑结构

• DOI: 10.21769/bioprotoc.1363
• 发表时间: 2014
• 作者: Torabi S;Plöchinger M;Meurer J
• 通讯作者: Meurer J

PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum[W]

• DOI: 10.1105/tpc.113.120444
• 发表时间: 2014-03
• 期刊: Plant Cell
• 影响因子: 11.6
• 作者: Salar Torabi;Pavan Umate;Nikolay Manavski;Magdalena Plöchinger;Laura Kleinknecht;Hanumakumar Bogireddi