Structural role of photosystem II supercomplexes in thylakoid membrane stacking

光系统II超复合物在类囊体膜堆积中的结构作用

• 批准号: 1945068
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1945068
• 关键词: Structural; role; photosystem; II; supercomplexes

Life on earth depends on photosynthesis, the source of all of our food, oxygen and most of our energy. The early steps of photosynthesis involve trapping of solar energy by electron transfer reactions in the photosynthetic membrane. Our recent studies have revealed an unexpected role for the membrane protein photosystem II (PSII) supercomplexes in mediating the stacking of chloroplast thylakoid membranes. Membrane stacking instigates the spatial segregation of the slow excitation energy trap PSII from the faster trap photosystem I (PSI), and promotes energy transfer among PSII units both of which are crucial for the efficiency of photosynthesis. For the first time we have biochemically-isolated a unique stacked form of the PSII supercomplex which will allow us to investigate this critical feature of the process. This project will make use of the latest advances in structural and functional microscopies to characterise this PSII supercomplex and understand how and why thylakoid membranes stack in molecular detail.

地球上的生命依赖于光合作用，它是我们所有食物、氧气和大部分能量的来源。光合作用的早期步骤包括通过光合膜中的电子转移反应捕获太阳能。我们最近的研究揭示了膜蛋白光系统II（PSII）的超复合物介导的叶绿体类囊体膜的堆叠意想不到的作用。膜堆积促使慢激发能陷阱PSII从快陷阱光系统I（PSI）的空间分离，并促进PSII单元之间的能量传递，这两者对于光合作用的效率是至关重要的。这是我们第一次从生物化学上分离出PSII超复合物的独特堆叠形式，这将使我们能够研究该过程的这一关键特征。该项目将利用结构和功能显微镜的最新进展来研究这种PSII超复合物，并了解类囊体膜如何以及为什么在分子细节上堆叠。

项目成果

Single-molecule study of redox control involved in establishing the spinach plastocyanin-cytochrome bf electron transfer complex

建立菠菜质体蓝素-细胞色素 bf 电子转移复合物的氧化还原控制的单分子研究

• DOI: 10.1016/j.bbabio.2019.06.013
• 发表时间: 2019
• 期刊: Biochimica et Biophysica Acta (BBA) - Bioenergetics
• 作者: Mayneord G