Novel players of the cell wall integrity sensing pathway controlled by the ANXUR Receptor-like kinases.

由 ANXUR 受体样激酶控制的细胞壁完整性传感通路的新参与者。

• 批准号: 267437986
• 负责人: Privatdozent Dr. Aurélien Boisson-Dernier, Ph.D.
• 金额: --
• 依托单位: Institut Sophia Agrobiotech - INRAE PACA
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/267437986?language=en
• 关键词: Novel; players; cell; wall; integrity

Plant cells are directing the synthesis/deposition of an extracellular matrix rigid yet dynamic known as the cell wall (CW) that fulfils various key functions during development. The cells synthesize primary CWs during growth, which depends on the balance between loosening/deformation of the pre-existing CW and continuous secretion of new material. This essential coordination requires the cell to be informed of any environmental perturbations that impact its CW properties so as to avoid growth arrest or rupture. To circumvent these catastrophic scenarios, it has become increasingly evident that plant cells have developed CW integrity (CWI) sensing mechanisms to relay information about CW performance to the internal growth machinery. Despite its importance in plant development and growth, our understanding of the signalling mechanisms between the CW and the intracellular machineries remains however meagre.The receptor-like kinases (RLKs) of the Catharanthus roseus RLK1-like (CrRLK1L) family have recently emerged as candidates for coordinating cell growth, cell-cell communication, and CW remodelling during development (Lindner, et al., 2012). Interestingly, two redundant CrRLK1L members named ANXUR1 (ANX1) and ANX2 function in the control of CW integrity (CWI) in pollen tubes (PTs), the male gametophyte of flowering plants. Pollen carrying mutations in both receptors burst shortly after germination, preventing them from fertilizing female gametophytes thereby leading to sterility (Boisson-Dernier, et al., 2009). Recently, ANX-RLKs were shown to regulate H2O2 production, Ca2+ homeostasis and secretion through NADPH oxidases to achieve steady growth without losing integrity (Boisson-Dernier, et al., 2013).To identify new players of this largely unexplored pathway, a anx1;anx2 sterility suppressor screen was performed and led to the identification of suppressors with improved fertility due to rescue of anx1;anx2 PT growth. For our first two suppressors, mapping by next-generation sequencing identified non-synonymous mutations in key functional domains of a novel receptor-like cytoplasmic kinase (RLCK) and a protein Ser/Thr phosphatase (PPP), respectively. Both the RLCK and the PPP are pollen-preferentially expressed and were named MARIS (MRI) and ATUNIS1 (AUN1) according to Etruscan deities of fertility and rebirth, respectively. Through an interdisciplinary proposal that includes state-of-the-art techniques in molecular and cell biology, genetics and biochemistry we propose to unravel the role of MRI and AUN1 in the ANX-RLK dependent CWI sensing pathway.

植物细胞指导细胞外基质的合成/沉积，该细胞外基质刚性而动态，称为细胞壁（CW），在发育过程中履行各种关键功能。细胞在生长过程中合成初级CW，这取决于先前存在的CW的松动/变形与新材料的持续分泌之间的平衡。这种重要的协调要求细胞了解影响其 CW 特性的任何环境扰动，以避免生长停滞或破裂。为了避免这些灾难性的情况，越来越明显的是，植物细胞已经开发出 CW 完整性 (CWI) 传感机制，将有关 CW 性能的信息传递给内部生长机制。尽管它在植物发育和生长中很重要，但我们对 CW 和细胞内机器之间的信号传导机制的了解仍然很少。长春花 RLK1 样 (CrRLK1L) 家族的受体样激酶 (RLK) 最近已成为协调细胞生长、细胞间通讯和发育过程中 CW 重塑的候选者（Lindner 等人， 2012）。有趣的是，两个名为 ANXUR1 (ANX1) 和 ANX2 的冗余 CrRLK1L 成员在控制花粉管 (PT)（开花植物的雄配子体）中的 CW 完整性 (CWI) 中发挥作用。两种受体中携带突变的花粉在发芽后不久就会爆发，阻止它们使雌配子体受精，从而导致不育（Boisson-Dernier 等，2009）。最近，ANX-RLK 被证明可以通过 NADPH 氧化酶调节 H2O2 产生、Ca2+ 稳态和分泌，以在不失去完整性的情况下实现稳定生长（Boisson-Dernier 等人，2013）。为了识别这一很大程度上未经探索的途径的新参与者，进行了 anx1;anx2 不育抑制子筛选，并导致识别出因拯救 anx1;anx2 PT 而提高生育力的抑制子 生长。对于我们的前两个抑制因子，通过新一代测序进行作图，分别识别出新型受体样细胞质激酶（RLCK）和蛋白质丝/苏氨酸磷酸酶（PPP）关键功能域中的非同义突变。 RLCK 和 PPP 均在花粉中优先表达，根据伊特鲁里亚生育神和重生神分别命名为 MARIS (MRI) 和 ATUNIS1 (AUN1)。通过一项包括分子和细胞生物学、遗传学和生物化学领域最先进技术的跨学科提案，我们建议阐明 MRI 和 AUN1 在 ANX-RLK 依赖的 CWI 传感通路中的作用。

项目成果

Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy.

使用 Yellow Cameleon 和共焦激光扫描显微镜对野生型和 NADPH 氧化酶缺陷突变体花粉管中的 Ca2 动态进行成像

• DOI: 10.1007/978-1-4939-7286-9_10
• 发表时间: 2017
• 期刊: Methods in molecular biology
• 作者: Franck;Westermann;Boisson-Dernier
• 通讯作者: Boisson-Dernier

RALF4/19 peptides interact with LRX proteins to control pollen tube growth in Arabidopsis

• DOI: 10.1126/science.aao5467
• 发表时间: 2017-12-22
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Mecchia, Martin A.;Santos-Fernandez, Gorka;Grossniklaus, Ueli
• 通讯作者: Grossniklaus, Ueli

Receptor-like cytoplasmic kinase MARIS functions downstream of CrRLK1L-dependent signaling during tip growth

• DOI: 10.1073/pnas.1512375112
• 发表时间: 2015-09-29
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Boisson-Dernier, Aurelien;Franck, Christina Maria;Grossniklaus, Ueli
• 通讯作者: Grossniklaus, Ueli

The Protein Phosphatases ATUNIS1 and ATUNIS2 Regulate Cell Wall Integrity in Tip-Growing Cells

• DOI: 10.1105/tpc.18.00284
• 发表时间: 2018-08-01
• 期刊: PLANT CELL
• 影响因子: 11.6
• 作者: Franck, Christina Maria;Westermann, Jens;Boisson-Dernier, Aurelien
• 通讯作者: Boisson-Dernier, Aurelien