Structural basis of translation control in eukaryotic 80S ribosomes by phosphorylation of eS6 (A06*)

作为SFB740的一部分，我们的目标是确定eS6磷酸化影响核糖体靶mrna选择的机制。作为进一步的假设，我们将探讨翻译是否以及如何由es6尾部修饰模式（或简单的开/关机制）控制，即是否存在与CTD控制转录或组蛋白代码假设等效的核糖体。这将突出翻译和转录之间调节的相似性，并对我们对翻译控制的理解产生巨大影响。此外，通过定量交联/质谱（CLMS）和高分辨率CLMS，我们将技术引入蛋白质组装，这将允许在该联盟的其他研究项目中探测广泛的机制问题，特别是A04项目，M. Wahl， B05项目，T. Sommer和C07项目，O. Daumke。

As part of the SFB740 we aim to determine the mechanism by which eS6 phosphorylation influences target-mRNA selection of ribosomes. As further reaching hypothesis we will probe if and how translation is governed by eS6-tail modification patterns (or a simple on/off mechanism), i.e. if there is a ribosomal equivalent to CTD control of transcription or the histone code hypothesis. This would highlight a similarity of regulation between translation and transcription and have huge impact on our understanding of translational control. Moreover, with quantitative cross-linking/mass spectrometry (CLMS) and high-resolution CLMS we introduce technologies to protein assemblies that will allow probing a broad range of mechanistic questions in other research projects of this consortium, specifically Project A04, M. Wahl, Project B05, T. Sommer and Project C07, O. Daumke.