Extracellular matrix after acute cardiac ischemia: Mechanisms of hyaluronan-mediated protection (A08)

该项目旨在加深对透明质酸(HA)基质在心肌缺血中的有益作用的机制的了解。因此，RHAMM(透明质酸介导的运动受体)的作用将通过转基因小鼠来揭示。通过删除透明质酸酶1/-2或过度表达透明质酸合成酶2，进一步增加I/R后局部HA/HA信号的表达是否会改善心功能。此外，我们还探讨了T2 DM患者I/R后的愈合反应是否与心脏HA的合成有关。最后，化学交换饱和转移成像将被应用于大型动物心脏早期HA的监测，作为潜在的生物标志物。

The project aims to deepen the mechanistic understanding of the beneficial effects of hyaluronan (HA) matrix in cardiac ischemia. Therefore, the role of RHAMM (receptor of HA-mediated motility) will be unraveled using transgenic mice. It will be tested whether further increase of local HA/HA-signaling post-I/R by either deletion of hyaluronidases 1/-2 or overexpression of HA-synthase 2 will improve cardiac function. Furthermore, we ask whether the healing response post-I/R in T2DM is interrelated with cardiac HA synthesis. Finally, chemical exchange saturation transfer imaging will be applied to monitor early cardiac HA in large animals as a potential biomarker.