Fabrication of electrospun fibre mats with defined geometry and load profile

具有确定的几何形状和负载分布的电纺纤维垫的制造

• 批准号: 270149134
• 负责人: Professorin Dr.-Ing. Birgit Glasmacher
• 金额: --
• 依托单位: Institut für Mehrphasenprozesse (IMP)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/270149134?language=en
• 关键词: Fabrication; electrospun; fibre; mats; defined

Subproject 3 (SP3) focuses on the fabrication of fiber mats from polycaprolactone (PCL) via electrospinning. These fiber mats should have graded mechanical and geometric properties as well as have to withstand the native loading conditions. Approaches to fabricate fiber mats for in vivo studies in rats or sheep suffered from inappropriate mechanical properties of the scaffold. This issue is going to be solved in the second funding period by combining solution electrospinning with melt electrospinning. This combination allows for the fabrication of larger fiber sizes with appropriate mechanical properties. It enables the fabrication of scaffolds with load-bearing structures combined with structures mimicking the native extracellular matrix to enhance cell infiltration. The resulting scaffolds consists of a combination of micro- and nanofibers, which increases the specific surface. The specific surface is known to influence cell attachment, which is going to be investigated in SP 1. Cell adhesion forces of BM-MSCs will be measured via atomic force microscopy (AFM). Studies in the first funding period have shown the crystallinity of the polymer being one of the factors with high impact on the mechanical properties (results of SP4). The crystallinity can be influenced by changing the rotating velocity of the collector. It increases with increasing velocity. This effect as well as the blending with chitosan-g-polycaprolactone (SP4) will be used to tailor the mechanical properties of the scaffold during the next funding period. In addition, the degradation kinetics will be investigated in cooperation with SP 6. Here, mass loss as well as changes in the molecular structure (Raman spectroscopy) will be analyzed to further investigate the degradation process. The working plan of this subproject will result in a scaffold, which is tailored to the loading conditions in small and large animals. The performance of this scaffold is assessed in animal trials (SP8).

subproject 3（SP3）着重于通过静电纺丝从聚苯乙酮（PCL）制造纤维垫。这些纤维垫应具有分级的机械和几何特性，并必须承受天然载荷条件。用于在大鼠或绵羊中进行体内研究的纤维垫的方法，脚手架的机械性能不适。通过将溶液静电纺丝与融化静电纺丝结合在一起，将在第二个资金期间解决此问题。这种组合允许制造具有适当机械性能的较大纤维尺寸。它可以用承重结构与模仿天然细胞外基质的结构结合使用脚手架，从而增强细胞浸润。所得的支架由微纤维和纳米纤维的组合组合，从而增加了特定的表面。已知特异性表面会影响细胞附着，这将在SP 1中进行研究。将通过原子力显微镜（AFM）测量BM-MSC的细胞粘附力。在第一个资金期间的研究表明，聚合物的结晶度是对机械性能产生高影响的因素之一（SP4的结果）。结晶度可以通过改变收集器的旋转速度来影响。它随着速度的增加而增加。这种效果以及与壳聚糖-G-聚甲酰基（SP4）的混合将在下一个资金期间定制支架的机械性能。此外，将与SP 6合作研究降解动力学。在这里，将分析质量损失以及分子结构（拉曼光谱）的变化，以进一步研究降解过程。 该副本的工作计划将导致脚手架，该脚手架是针对大小动物的负载条件量身定制的。该支架的性能在动物试验（SP8）中进行了评估。