Fabrication of electrospun fibre mats with defined geometry and load profile

具有确定的几何形状和负载分布的电纺纤维垫的制造

• 批准号: 270149134
• 负责人: Professorin Dr.-Ing. Birgit Glasmacher
• 金额: --
• 依托单位: Institut für Mehrphasenprozesse (IMP)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/270149134?language=en
• 关键词: Fabrication; electrospun; fibre; mats; defined

Subproject 3 (SP3) focuses on the fabrication of fiber mats from polycaprolactone (PCL) via electrospinning. These fiber mats should have graded mechanical and geometric properties as well as have to withstand the native loading conditions. Approaches to fabricate fiber mats for in vivo studies in rats or sheep suffered from inappropriate mechanical properties of the scaffold. This issue is going to be solved in the second funding period by combining solution electrospinning with melt electrospinning. This combination allows for the fabrication of larger fiber sizes with appropriate mechanical properties. It enables the fabrication of scaffolds with load-bearing structures combined with structures mimicking the native extracellular matrix to enhance cell infiltration. The resulting scaffolds consists of a combination of micro- and nanofibers, which increases the specific surface. The specific surface is known to influence cell attachment, which is going to be investigated in SP 1. Cell adhesion forces of BM-MSCs will be measured via atomic force microscopy (AFM). Studies in the first funding period have shown the crystallinity of the polymer being one of the factors with high impact on the mechanical properties (results of SP4). The crystallinity can be influenced by changing the rotating velocity of the collector. It increases with increasing velocity. This effect as well as the blending with chitosan-g-polycaprolactone (SP4) will be used to tailor the mechanical properties of the scaffold during the next funding period. In addition, the degradation kinetics will be investigated in cooperation with SP 6. Here, mass loss as well as changes in the molecular structure (Raman spectroscopy) will be analyzed to further investigate the degradation process. The working plan of this subproject will result in a scaffold, which is tailored to the loading conditions in small and large animals. The performance of this scaffold is assessed in animal trials (SP8).

子项目3 （SP3）侧重于通过静电纺丝从聚己内酯（PCL）制备纤维垫。这些纤维垫应该有分级的机械和几何性能，以及必须承受自然载荷条件。在大鼠或羊的体内研究中，纤维垫的制备方法存在支架机械性能不合适的问题。在第二个资助期内，将采用溶液静电纺丝与熔体静电纺丝相结合的方法来解决这一问题。这种组合允许制造具有适当机械性能的更大尺寸的纤维。它可以制造具有承重结构和模拟天然细胞外基质结构相结合的支架，以增强细胞浸润。所得到的支架由微纤维和纳米纤维的组合组成，这增加了比表面。已知特定表面会影响细胞附着，这将在sp1中进行研究。通过原子力显微镜（AFM）测量脑基质间充质干细胞的细胞粘附力。在第一个资助期的研究表明，聚合物的结晶度是对机械性能有很大影响的因素之一（SP4的结果）。通过改变收集器的旋转速度可以影响结晶度。它随着速度的增加而增加。这种效应以及与壳聚糖-g-聚己内酯（SP4）的混合将在下一个资助期内用于定制支架的机械性能。此外，还将与sp6合作研究其降解动力学。在这里，将分析质量损失以及分子结构的变化（拉曼光谱），以进一步研究降解过程。该子项目的工作计划将产生一个脚手架，该脚手架适合小型和大型动物的加载条件。在动物试验中评估了这种支架的性能（SP8）。