Aberration correction for real-time measurements in adaptive confocal microscopy

自适应共焦显微镜实时测量的像差校正

基本信息

项目摘要

Progress in the in vivo investigation of biological tissue essentially relies on microscopy with ever increasing spatial and temporal resolution. In the present proposal, we address this challenge by aiming to enhance the spatial resolution confocal laser scanning microscopy using novel adaptive optical elements and control techniques. To produce a three dimensional image, confocal microscopy scans the volume with a focused laser beam, typically by moving optical elements and using piezo or galvo mirrors where the speed is limited by mass and inertia. This may further result in motion artefacts and requires a bulky set-up, limiting the potential for miniaturization. The spot size, and hence the resolution, can - in principle - be minimized using microscope objectives with a high numerical aperture. As they are optimized for a single focal plane with a limited field, however, scanning results in significant system-induced aberrations in addition to the sample-induced aberrations and hence limits the achievable resolution. To overcome these problems, one can use adaptive elements both for aberration correction and for motion-free scanning.Our aim is the development of an adaptive confocal microscope that uses ideally only two (one lens and one prism) adaptive optical elements to provide fast three dimensional scanning and real-time aberration correction for diffraction-limited imaging over the whole field of view. For this purpose, we will develop novel adaptive lenses that combine axial scanning and aberration correction, including geometric (defocus, spherical, astigmatism, coma) and chromatic aberrations. Similarly, we will preform the lateral scans with novel bi-axial achromatic adaptive prisms. In contrast to scanning with galvo mirrors, this allows for a more compact collinear geometry. Using these adaptive optical elements, this novel smart microscope has a great potential for miniaturization and for the development of robust handheld systems. We will demonstrate potential applications and the enhanced imaging performance by investigating the effects of goitrogens in zebrafish embryos.
生物组织体内研究的进展本质上依赖于空间和时间分辨率不断提高的显微镜。在本提案中,我们通过使用新型自适应光学元件和控制技术来提高共焦激光扫描显微镜的空间分辨率来应对这一挑战。为了产生三维图像,共焦显微镜通常通过移动光学元件并使用压电镜或检流镜来使用聚焦激光束扫描体积,其中速度受到质量和惯性的限制。这可能进一步导致运动伪影并且需要庞大的设置,从而限制了小型化的潜力。原则上,使用具有高数值孔径的显微镜物镜可以最小化光斑尺寸,从而最小化分辨率。然而,由于它们针对具有有限视场的单个焦平面进行了优化,因此除了样品引起的像差之外,扫描还会导致显着的系统引起的像差,从而限制了可实现的分辨率。为了克服这些问题,可以使用自适应元件进行像差校正和无运动扫描。我们的目标是开发一种自适应共焦显微镜,理想情况下仅使用两个(一个镜头和一个棱镜)自适应光学元件来提供快速三维扫描和实时像差校正,从而在整个视场上进行衍射限制成像。为此,我们将开发新型自适应镜头,结合轴向扫描和像差校正,包括几何像差(散焦、球面、像散、慧差)和色差。同样,我们将使用新型双轴消色差自适应棱镜进行横向扫描。与使用振镜扫描相比,这可以实现更紧凑的共线几何形状。利用这些自适应光学元件,这种新型智能显微镜在小型化和开发稳健的手持系统方面具有巨大的潜力。我们将通过研究致甲状腺肿素对斑马鱼胚胎的影响来展示潜在的应用和增强的成像性能。

项目成果

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Professor Dr.-Ing. Jürgen W. Czarske其他文献

Professor Dr.-Ing. Jürgen W. Czarske的其他文献

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{{ truncateString('Professor Dr.-Ing. Jürgen W. Czarske', 18)}}的其他基金

Physical Layer Security of Multimode Optical Fiber Transmission Systems
多模光纤传输系统的物理层安全
  • 批准号:
    410148962
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Laser based tomographic measurement of the local acoustic impedance of overflowed liners (TOMLIM)
基于激光断层扫描测量溢出内衬的局部声阻抗 (TOMLIM)
  • 批准号:
    408927635
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Full-Field Laser Vibrometry for Combustion Diagnostics
用于燃烧诊断的全场激光测振法
  • 批准号:
    282277522
  • 财政年份:
    2016
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Flow investigations in liquid metals for crystal growth under the influence of a travelling magnetic field using a dual-plane ultrasound measurement system
使用双平面超声测量系统研究行进磁场影响下晶体生长的液态金属的流动
  • 批准号:
    269790734
  • 财政年份:
    2015
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Adaptive Lasersysteme mit Wellenfrontregelung und Phasenkonjugation für Strömungsmessungen bei Brechungsindexeffekten
具有波前控制和相位共轭的自适应激光系统,用于具有折射率效应的流量测量
  • 批准号:
    259811751
  • 财政年份:
    2014
  • 资助金额:
    --
  • 项目类别:
    Reinhart Koselleck Projects
Novel three dimensional shape measurement technique for fast rotating objects
用于快速旋转物体的新型三维形状测量技术
  • 批准号:
    235791889
  • 财政年份:
    2013
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Laser optical measurements of the acoustic particle velocity at bias flow liners with grazing flow
激光光学测量偏流衬管处掠流声粒子速度
  • 批准号:
    196686122
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Optische Untersuchung dynamischer Deformationen schnell drehender Rotoren
快速旋转转子动态变形的光学研究
  • 批准号:
    182207875
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Zeitlich hochauflösendes Doppler-Global-Velozimeter zur Turbulenzspektrenmessung
用于湍流谱测量的高分辨率多普勒全局速度计
  • 批准号:
    158620360
  • 财政年份:
    2010
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Neuartiger Laser-Doppler-Sensor zur Abstands- und Formvermessung bewegter Festkörper
创新的激光多普勒传感器,用于测量移动固体的距离和形状
  • 批准号:
    29343999
  • 财政年份:
    2006
  • 资助金额:
    --
  • 项目类别:
    Research Grants

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实时空气折射率自校正高速高精度激光雷达研究
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适用于实时、流媒体和广播视频应用的校验和辅助视频纠错
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