GRK 521: Protein-Protein Interactions during Intracellular Transport of Macromolecules

这个研究培训小组的目的是在一个跨学科的项目中阐明调节细胞内运输过程的控制机制。研究培训小组是医学和生物学院以及马克斯·普朗克生物物理化学和实验医学研究所的几个系的联合项目。新合成的大分子必须有靶点才能到达它们的最后一个细胞内隔室。研究生课程的项目首先涉及蛋白质、核糖核酸和病毒成分在细胞核和细胞质之间的运输，其次是蛋白质向内质网的转运，第三是内质网、高尔基体、溶酶体和质膜之间的囊泡运输的调节。这一点在哺乳动物细胞、植物细胞和酵母中都有研究。因此，该计划包括关于大分子和囊泡的分子运输以及蛋白质-蛋白质相互作用在调节这些过程中的作用的项目。使用的生物系统是哺乳动物和酵母细胞，以及植物和病毒。研究生定期开会讨论方法和结果。此外，参与实验室的负责人报告其实验室的活动情况。研究方法的理论基础研讨会和嘉宾讲者的演讲完成了课程。跨学科的方法和方法的范围也是实验室课程的基础，这些课程在三年课程的前半部分进行，介绍基础课程中没有教授的这些方法。这些实践的目的是获得与自己的项目没有直接关系的方法方面的经验，并了解参与小组的所有方法。

The aim of this research training group is within an interdisciplinary program the elucidation of the control mechanisms regulating intracellular transport processes. The research training group is a joint program of several departments of the medical and biological faculties and of the Max Planck Institutes for Biophysical Chemistry and of Experimental Medicine. Newly synthesized macromolecules must be targeted to reach their final intracellular compartment. The projects of the graduate program deal first with the transport of proteins, ribonucleic acids and viral components between nucleus and cytoplasm, secondly with the translocation of proteins into the endoplasmic reticulum, and thirdly, with the regulation of the vesicular transport between the endoplasmic reticulum, the Golgi apparatus, the lysosomes and the plasma membrane. This is studied in mammalian cells as well as in plant cells and in yeast. Thus, the program includes projects on the molecular transport of macromolecules and vesicles and on the role of protein-protein interactions in regulating these processes. The biological systems used are mammalian and yeast cells as well as plants and viruses. The graduate students have regular meetings for the discussion of methods and results. In addition, the heads of the participating laboratories report on the activities in their labs. Seminars of the theoretical basis of research methods and talks given by guest speakers complete the curriculum. The interdisciplinary approach and the spectrum of methods are also the basis of laboratory courses which take place in the first half of the 3-years period of the program and introduce to such methods that are not taught in basic curricula. The aim of these practicals is to gain experience in methods not directly related to the own project and to learn about the whole spectrum of methods of the participating groups.