Investigating the structural basis of human antibody stability

研究人类抗体稳定性的结构基础

• 批准号: DE160100608
• 负责人: Dr Mary Christie
• 金额: $ 23.84万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-03-31 至 2019-01-18
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE160100608
• 关键词: Investigating; structural; basis; human; antibody

This project plans to use protein engineering and X-ray crystallography to investigate the effects of stabilising mutations on antigen binding and the antibody-antigen interaction. Monoclonal antibodies are high-affinity reagents that have transformed the study of biological processes. However, antibodies often display inherent instability, which limits applicability. Mutations have recently been identified that render human antibodies resistant to aggregation. Preliminary data indicates that stabilising mutations improves the biophysical properties of monoclonals without affecting the native antibody structure. The project aims to provide detailed insights into the molecular basis of antibody stability.

该项目计划使用蛋白质工程和x射线晶体学来研究稳定突变对抗原结合和抗体-抗原相互作用的影响。单克隆抗体是一种高亲和力的试剂，已经改变了生物过程的研究。然而，抗体通常表现出固有的不稳定性，这限制了其适用性。最近发现的突变使人类抗体对聚集具有抵抗力。初步数据表明，稳定突变在不影响天然抗体结构的情况下改善了单克隆抗体的生物物理特性。该项目旨在提供抗体稳定性的分子基础的详细见解。