INVESTIGATING THE STRUCTURAL BASIS OF IME2 REGULATION AND SUBSTRATE SPECIFICITY
研究 IME2 调节和底物特异性的结构基础
基本信息
- 批准号:7957836
- 负责人:
- 金额:$ 0.14万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-09-01 至 2010-08-31
- 项目状态:已结题
- 来源:
- 关键词:AnimalsBiological ModelsBiologyCell DeathCellsChromosomesComputer Retrieval of Information on Scientific Projects DatabaseCongenital AbnormalityDevelopmentDown SyndromeEnzymesFemaleFundingFungal GenomeGrantHumanHuman DevelopmentInfertilityInstitutionInvestigationLeadModelingProcessPropertyProteinsRegulationReproductionResearchResearch PersonnelResourcesSourceSubstrate SpecificityTimeUnited States National Institutes of Healthbasecell typedesignegginsightmaleprogramssperm cellthree dimensional structure
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Cells that make up an animal or human normally divide a specific number of times before they stop and take on properties that allow them to perform specific functions. One particular cell type ultimately forms sperm in males and egg cells in females; these cells are essential for reproduction. We are using a model system to investigate factors that are essential for growing cells to initiate the program leading to sperm or eggs. A protein referred to as Ime2 (Mak1 in humans) is responsible for promoting the process of sperm and egg formation. Ime2 performs this function by modifying the activity of a specific set of other proteins at just the right time during development. Loss of Ime2 activity leads to infertility, and improper regulation of Ime2 can lead to chromosome damage In humans this damage might result in birth defects such as Downs Syndrome. Additionally, if Ime2 activity is induced in growing cells it can lead to cell death. We are exploring the mechanisms that regulate Ime2 activity by modeling the 3-dimensional structure of the protein to gain insight into how it identifies the proteins that it regulates. Additionally this investigation will lead to new insights regarding how Ime2 activity is regulated. By discovering the details of how this enzyme functions, what other proteins it regulates, and how it accumulates in the right place at the right time, we hope to gain a deeper understanding of human development, and be able to design rational strategies to overcome some types of infertility.
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者所在的机构。
组成动物或人类的细胞通常会分裂特定的次数,然后停止分裂,并具有允许它们执行特定功能的特性。一种特殊的细胞类型最终形成男性的精子和女性的卵细胞;这些细胞对生殖至关重要。我们正在使用一个模型系统来研究细胞生长所必需的因素,以启动导致精子或卵子的程序。一种被称为Ime 2(人类中的Mak 1)的蛋白质负责促进精子和卵子形成的过程。Ime 2通过在发育过程中的正确时间修改一组特定的其他蛋白质的活性来执行这一功能。Ime 2活性的丧失导致不育,并且Ime 2的不适当调节可导致染色体损伤。在人类中,这种损伤可导致出生缺陷,例如唐斯综合征。此外,如果在生长的细胞中诱导Ime 2活性,则其可导致细胞死亡。我们正在探索通过模拟蛋白质的三维结构来调节Ime 2活性的机制,以深入了解它如何识别它所调节的蛋白质。此外,这项研究将导致关于Ime 2活性如何调节的新见解。通过发现这种酶如何发挥作用的细节,它调节的其他蛋白质以及它如何在正确的时间在正确的地方积累,我们希望能够更深入地了解人类发育,并能够设计合理的策略来克服某些类型的不孕症。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DAVID I. STUART其他文献
DAVID I. STUART的其他文献
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{{ truncateString('DAVID I. STUART', 18)}}的其他基金
IDENTIFYING MEIOSIS-SPECIFIC SUBSTRATES OF CLB5-CDK1
鉴定 CLB5-CDK1 减数分裂特异性底物
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- 资助金额:
$ 0.14万 - 项目类别:
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