Mechanisms of early amyloid formation and the confining influence of macromolecular crowding (B12)

早期淀粉样蛋白形成的机制和大分子拥挤的限制影响（B12）

• 批准号: 276254115
• 金额: --
• 依托单位: Abteilung Allgemeine Biochemie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/276254115?language=en
• 关键词: Mechanisms; early; amyloid; formation; confining

This project aims to gain a more detailed understanding of the early prefibrillar growth process of amyloidic aggregates and the influence of a constraining environment. In order to reveal the initial pathways of aggregation and disassembly, size distributions of small transient aggregates (named oligomers) are experimentally studied along the time course of fibrillation. Having started with investigations of disease relevant amyloid peptides in the last funding period, we plan to compare the obtained results to the functional amyloid PTH in the next funding period. In addition, macromolecular crowding will be used to reveal the impact of an outer confinement and to discuss the importance of intermolecular interactions in order to test current models of nucleation and growth.

本项目旨在更详细地了解淀粉酶聚集体的早期前原纤维生长过程和约束环境的影响。为了揭示聚集和分解的初始途径，实验研究了小的瞬时聚集体（命名为寡聚体）的尺寸分布沿着原纤化的时间过程。在上一个资助期开始研究疾病相关的淀粉样肽后，我们计划在下一个资助期将获得的结果与功能性淀粉样PTH进行比较。此外，大分子拥挤将被用来揭示外部限制的影响，并讨论分子间相互作用的重要性，以测试目前的成核和生长模型。