Deciphering the role of Arabidopsis SERRATE in transcription and splicing

破译拟南芥 SERRATE 在转录和剪接中的作用

• 批准号: 276820861
• 负责人: Professor Dr. Sascha Laubinger
• 金额: --
• 依托单位: Abteilung Pflanzengenetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/276820861?language=en
• 关键词: Deciphering; role; Arabidopsis; SERRATE; transcription

Gene expression can be controlled a various levels, e.g at the level of transcription, RNA processing or translation. The essential plant protein SERRATE (SE) is an important regulator of gene expression. The SE protein acts as a scaffold protein that mediates interactions with other proteins and RNAs. Mechanistically, SE enhances transcription by directly binding to the chromatin of its target genes, it promotes primary microRNA processing, it facilitates splicing of a subset of introns and it affects RNA stability. Furthermore, the metazoan homologue of SE, ARS2, has been implicated in additional RNA processing events such as 3’end processing or RNA transport. In this proposal, we will focus on two distinct function of SE in regulating gene expression: Firstly, while SE’s function in promoting transcription is established, we observed that SE represses transcription by an yet unknown mechanism. Secondly, SE forms complexes with the spliceosomal U1 snRNP complex, but this interaction seems to be dispensable for SE’s function in splicing. We will investigate alternative functions of the interaction between SE and the U1 snRNP, which might have important implication for regulating gene expression in plants.

基因表达可以在各种水平上控制，例如在转录、RNA加工或翻译水平上。必需的植物蛋白SERATE（SE）是基因表达的重要调节剂。SE蛋白充当介导与其他蛋白质和RNA相互作用的支架蛋白。从机制上讲，SE通过直接结合其靶基因的染色质来增强转录，它促进初级microRNA加工，它促进内含子子集的剪接，并且它影响RNA稳定性。此外，SE的后生动物同源物ARS 2涉及额外的RNA加工事件，如3 '末端加工或RNA转运。在这个建议中，我们将集中在两个不同的功能，SE在调节基因表达：首先，虽然SE的功能，在促进转录的建立，我们观察到，SE抑制转录的一个未知的机制。其次，SE与剪接体U1 snRNP复合物形成复合物，但这种相互作用似乎与SE的剪接功能无关。我们将研究SE和U1 snRNP之间相互作用的替代功能，这可能对调节植物基因表达具有重要意义。