Molecular and biochemical characterization of SERRATE and the nuclear cap-binding complex

SERRATE 和核帽结合复合物的分子和生化特征

• 批准号: 84149828
• 负责人: Professor Dr. Sascha Laubinger
• 金额: --
• 依托单位: Abteilung Pflanzengenetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/84149828?language=en
• 关键词: Molecular; biochemical; characterization; SERRATE; nuclear

Biogenesis of plant miRNAs requires several proteins (like DCL1, SERRATE and HYL1) that release mature miRNAs from longer precursor transcripts. I could show that the nuclear cap-binding complex, which consists of ABH1 and CBP20, is also necessary for proper processing of these longer precursor transcripts. Furthermore, I found that SERRATE and the nuclear cap-binding complex have additional functions, like splicing, that are distinct from other factors of the miRNA-biogenesis machinery. The main goal of the proposed project isto identify the molecular and biochemical functions of the SERRATE and the cap-binding complex in miRNA processing and splicing,to identify RNAs that are targeted by their functions,and to identify protein complexes that are formed by SERRATE and the capbinding complex.

植物miRNAs的生物发生需要几种蛋白质（如DCL1，SERRATE和HYL1）从较长的前体转录物中释放成熟的miRNAs。我可以证明，由ABH 1和CBP 20组成的核帽结合复合物对于这些较长的前体转录物的正确加工也是必要的。此外，我发现SERRATE和核帽结合复合物具有额外的功能，如剪接，这与miRNA生物发生机制的其他因素不同。该项目的主要目标是鉴定SERRATE和帽结合复合物在miRNA加工和剪接中的分子和生物化学功能，鉴定其功能靶向的RNA，并鉴定由SERRATE和帽结合复合物形成的蛋白质复合物。

项目成果

Dual roles of the nuclear cap-binding complex and SERRATE in pre-mRNA splicing and microRNA processing in Arabidopsis thaliana

• DOI: 10.1073/pnas.0802493105
• 发表时间: 2008-06-24
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Laubinger, Sascha;Sachsenberg, Tirno;Weigel, Detlef
• 通讯作者: Weigel, Detlef

Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome

• DOI: 10.1073/pnas.1012891107
• 发表时间: 2010-10-12
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Laubinger, Sascha;Zeller, Georg;Weigel, Detlef
• 通讯作者: Weigel, Detlef