Molecular and biochemical characterization of SERRATE and the nuclear cap-binding complex

植物mirna的生物发生需要几种蛋白质（如DCL1、SERRATE和HYL1）从较长的前体转录物中释放成熟的mirna。我可以证明核帽结合复合体，由ABH1和CBP20组成，也是正确处理这些较长的前体转录本所必需的。此外，我发现SERRATE和核帽结合复合体具有额外的功能，如剪接，这与mirna生物发生机制的其他因素不同。拟建项目的主要目标是鉴定SERRATE和帽结合复合体在miRNA加工和剪接中的分子和生化功能，鉴定其功能靶向的rna，鉴定由SERRATE和帽结合复合体形成的蛋白复合物。

Biogenesis of plant miRNAs requires several proteins (like DCL1, SERRATE and HYL1) that release mature miRNAs from longer precursor transcripts. I could show that the nuclear cap-binding complex, which consists of ABH1 and CBP20, is also necessary for proper processing of these longer precursor transcripts. Furthermore, I found that SERRATE and the nuclear cap-binding complex have additional functions, like splicing, that are distinct from other factors of the miRNA-biogenesis machinery. The main goal of the proposed project isto identify the molecular and biochemical functions of the SERRATE and the cap-binding complex in miRNA processing and splicing,to identify RNAs that are targeted by their functions,and to identify protein complexes that are formed by SERRATE and the capbinding complex.