Neuro MRE (II): Correlation of histology and changes in viscoelastic properties of the brain in animal models

Neuro MRE（II）：动物模型中大脑组织学与粘弹性特性变化的相关性

• 批准号: 276879121
• 负责人: Privatdozent Dr. Jürgen Braun
• 金额: --
• 依托单位: Experimental and Clinical Research Center (ECRC)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/276879121?language=en
• 关键词: Neuro; MRE; II; Correlation; histology

Elastography is the image-based detection of the mechanical properties of biological soft tissues for medical diagnosis. Elastography of the liver has already become an established clinical modality for the noninvasive staging of hepatic fibrosis. In contrast, the use of elastography for brain imaging is still limited despite initial success of magnetic resonance elastography (MRE) in demonstrating the high sensitivity of neuronal viscoelastic constants to disease.The goal of the proposed project is to test and ultimately establish viscoelasticity as a quantitative biomarker for neuroinflammatory and neurodegenerative processes by using MRE in mouse models. The following mouse models will be investigated: (i) Experimental autoimmune encephalitis(EAE) as a model of neuroinflammation induced by multiple sclerosis, and (ii) 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) as an accepted model for enhanced adult neurogenesis and neurodegeneration in Parkinson's disease MRE combined with extensive histological investigations will enable us to reveal and systematically analyze disease-related alterations of the viscoelastic matrix of neural tissue, specifically with regard to the following mechanisms: (i) demyelination, (ii) glial activation and distribution of activation patterns, (iii) changes in the extracellular matrix due to inflammatory mechanisms such as lymphocytic infiltration or endothelial alterations, and (iv) proliferation, differentiation, and integration of neurons into the tissue matrix. By addressing these issues, the proposed project is pivotal for the validation and deeper understanding of MRE in humans, which will be investigated in a parallel project. Moreover, Neuro MRE in the mouse serves as the underpinning for any other application of elastography as a structure-sensitive imaging modality.

弹性成像是基于图像的检测生物软组织用于医学诊断的机械性能。肝脏的弹性成像已经成为肝纤维化无创分期的已建立临床方式。相比之下，尽管磁共振弹性学（MRE）在证明神经元粘弹性常数对疾病的高灵敏度方面的最初成功，但该项目的目的是测试并最终确定粘弹性作为一种定量的神经毒素性和神经层的模型，但最终建立了粘弹性，但最终建立粘弹性的方法是使用粘弹性，该目标是使用粘弹性来建立粘弹性，但使用粘弹性来建立粘弹性，但使用粘弹性来建立粘弹性，该目标是使用粘弹性，但通过使用NeuroDeproces来建立粘弹性。 The following mouse models will be investigated: (i) Experimental autoimmune encephalitis(EAE) as a model of neuroinflammation induced by multiple sclerosis, and (ii) 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) as an accepted model for enhanced adult neurogenesis and neurodegeneration in Parkinson's disease MRE combined with extensive histological investigations将使我们能够揭示并系统地分析神经组织粘弹性基质的改变，特别是在以下机制方面：（i）脱髓鞘，（ii）激活模式的神经胶质激活和分布，（iii）因炎症机制而导致的炎症机制的变化（iii），淋巴细胞和淋巴细胞化的变化（或分化和神经元整合到组织基质中。通过解决这些问题，拟议的项目对于人类对MRE的验证和更深入的了解至关重要，这将在一个并行项目中进行调查。此外，小鼠中的Neuro MRE是任何其他应用弹性造影作为结构敏感成像方式的基础。