Mixed micellar systems in continuous countercurrent extraction columns for biocatalytic reactive separations

重点介绍了表面活性剂双水相体系在生物催化反应萃取中的应用。到目前为止，基于表面活性剂的双水相体系已被广泛应用于废水中金属离子、蛋白质、生物材料、有机化合物和有毒芳香族化合物的提取和纯化。利用连续转盘接触器成功地实现了第一套连续多级装置的放大。重要的是，本申请组实现了表面活性剂双水相体系在搅拌连续逆流萃取塔中的应用。基于表面活性剂的双水相体系为生物分子提供了温和的条件，这些生物分子对有机溶剂和高温敏感。因此，使用它们来原位去除产物以提高转化率对于生物催化和生物转化过程中的环境友好性特别感兴趣。此外，有机溶剂的缺乏为大多数生物催化剂创造了一个方便的环境。已经在批量实验中研究了胶束体系中的生物催化反应。然而，应用表面活性剂双水相体系的连续反应过程到目前为止还没有被研究过。本项目的主要目的是证明连续多级胶束萃取作为生物催化剂(酶反应)的反应分离过程的原理适用性。因此，应确定表面活性剂-添加剂混合体系的组成对连续萃取过程效率的关键影响。用混合表面活性剂体系萃取分子和电离溶质时，应特别注意生物催化剂对表面活性剂的稳定性和萃取条件。

This proposal is focused on the application of surfactant based aqueous two-phase systems for the reactive extraction with biocatalyst. Up to now, surfactant based aqueous two-phase systems have been used in various applications such as extraction and purification of metal ions, proteins, biomaterials, organic compounds and toxic aromatics from wastewater. The first scale-up to continuous multistage units was successfully realized by means of a continuous rotating disc contactor. Importantly, the application of aqueous surfactant two-phase systems in a stirred continuous countercurrent extraction column was achieved in the group of the applicant. Surfactant based aqueous two-phase systems systems provide mild conditions for biomolecules which are sensitive to organic solvents and high temperatures. Thus, their use for an in-situ product removal to increase conversion is especially interesting for biocatalytic and biotransformation processes with regard to environmental friendliness. Furthermore, the absence of organic solvents creates a convenient environment for most biocatalysts. A biocatalytic reaction in micellar systems was already studied in batch experiments. However, a continuous reactive process applying aqueous surfactant two-phase systems was not investigated so far. The main goal of this project is to prove the principle applicability of continuous multistage micellar extraction in a column as a reactive separation process for biological catalysts (enzymatic reactions). Thereby the key influences of the composition of mixed surfactant-additive systems on the efficiency of the continuous extraction process should be identified. The extraction of both molecular and ionized solutes with mixed surfactant systems should be established with the special focus on the stability of the biocatalysts towards surfactants and extraction conditions.