The role of PILMACs and of the genes bubbles and schwächling during muscle development in Drosophila melanogaster

PILMACs 以及气泡和弱化基因在果蝇肌肉发育过程中的作用

• 批准号: 27889892
• 负责人: Professorin Dr. Renate Renkawitz-Pohl
• 金额: --
• 依托单位: Arbeitsgruppe Kommunikation und Dynamik tierischer Zellen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/27889892?language=en
• 关键词: role; PILMACs; genes; bubbles; schw

During Drosophila embryogenesis, the muscle pattern is established and cell-cell fusion leads to multinucleated myotubes. Fusion-competent myoblasts (FCMs) fuse to founder cells (FCs)/growing myotubes. With Ribokinase (entry into ATP synthesis), we identified a new essential biochemical pathway for myogenesis with respect to Founder Cell (FC) determination and fusion. Looking at the individual site of fusion, FuRMASs are established, transient cell adhesion structures with signalling properties. Here, cell adhesion molecules, signalling molecules, F- actin, and its regulators do accumulate. At the ultrastructural level the individual fusion site is characterised by a cloud of vesicles at the opposing membranes over an area of 1mm2, named prefusion complex. Importantly, we identified two new components of the FuRMAS: 1) Tetraspanins as candidates for clustering of fusion-relevant molecules at the opposing membranes, and 2) Kosh, an EF hand, putatively Ca2+-binding protein. As a likely candidate for membrane bending during fusion, we identified a gene, which is predicted to encode a C2 domain protein with two putative transmembrane domains. Our central goals are the functional analysis of these newly identified fusion-relevant molecules and their integration into the fusion-relevant signalling cascades with respect to the topology of the FuRMASs.

在果蝇胚胎发育过程中，肌肉模式建立，细胞-细胞融合导致多核肌管。有融合能力的成肌细胞（FCM）融合到创始细胞（FC）/生长肌管。利用核糖激酶（进入ATP合成），我们确定了一个新的基本的生化途径，肌生成方面的方正细胞（FC）的确定和融合。观察融合的各个位点，建立了FuRMASs，具有信号传导特性的瞬时细胞粘附结构。在这里，细胞粘附分子，信号分子，F-肌动蛋白，和它的调节剂确实积累.在超微结构水平上，单个融合部位的特征是在1 mm 2面积上的相对膜上有一团囊泡，称为融合前复合体。重要的是，我们鉴定了FuRMAS的两个新组分：1）四跨膜蛋白作为融合相关分子在相对膜处成簇的候选物，和2）科什，EF手，puridine Ca 2+结合蛋白。作为融合过程中膜弯曲的可能候选者，我们确定了一个基因，该基因被预测编码具有两个推定跨膜结构域的C2结构域蛋白。我们的中心目标是这些新发现的融合相关分子的功能分析和融合相关的信号级联的FuRMASs的拓扑结构方面的整合。

项目成果

Multinucleated smooth muscles and mononucleated as well as multinucleated striated muscles develop during establishment of the male reproductive organs of Drosophila melanogaster.

• DOI: 10.1016/j.ydbio.2012.07.022
• 发表时间: 2012-10
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Loreen Susic-Jung;Christina Hornbruch-Freitag;Jessica Kuckwa;K. Rexer;U. Lammel;R. Renkawitz-Pohl