Activity-dependent development of precise temporal coding at calyceal synapses

了解早期动作电位放电如何促进神经网络的发育是发育神经生理学面临的主要挑战之一。在最初的发育过程之后，成熟神经元回路中的有模式的、独立于感觉的活动被认为指导着精确感觉图谱的形成。我们假设，不仅是听前活动的活动，而且是听前活动的模式，决定了听觉脑干中巨大的帽状突触的突触传递的发展。我们评估了活动模式在听觉发作前具有泊松样放电的小鼠模型(P2X2/P2X3Dbl-/-)中，在保持突触的终球和花瓣成熟中所起的作用。在不同发育阶段条件细胞特异性毛细胞消融的小鼠模型中，研究了早期听觉经验的贡献(Pou4f3DTR/)。实验设计用于体内和切片电生理学的互补使用，以评估通过系统声刺激进行的功能处理，并揭示潜在的细胞机制。

Understanding how early action potential discharges contribute to the development of neuronal networks is one of the major challenges in developmental neurophysiology. Following initial developmental processes, the patterned, sensory-independent activity in maturing neuronal circuits is believed to guide the formation of precise sensory maps. We hypothesize that not just the activity, but the pattern of prehearing activity, determines the development of synaptic transmission at giant calyceal synapses in the auditory brainstem. We assess the role of activity pattern in maturation of the endbulb- and calyx of Held-synapses in a mice model with Poisson-like firing before hearing onset (P2X2/P2X3Dbl-/-). The contribution of early auditory experience is investigated in a mouse model with conditional cell-specific hair cell ablation at different developmental stages (Pou4f3DTR/+). Experiments are designed for complementary use of in vivo- and slice-electrophysiology to assess functional processing through systemic acoustic stimulation and reveal underlying cellular mechanisms.