Activity-dependent development of precise temporal coding at calyceal synapses

肾盏突触精确时间编码的活动依赖性发育

• 批准号: 279508240
• 负责人: Professor Dr. Ivan Milenkovic
• 金额: --
• 依托单位: Abteilung für Physiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/279508240?language=en
• 关键词: Activity; dependent; development; precise; temporal

Understanding how early action potential discharges contribute to the development of neuronal networks is one of the major challenges in developmental neurophysiology. Following initial developmental processes, the patterned, sensory-independent activity in maturing neuronal circuits is believed to guide the formation of precise sensory maps. We hypothesize that not just the activity, but the pattern of prehearing activity, determines the development of synaptic transmission at giant calyceal synapses in the auditory brainstem. We assess the role of activity pattern in maturation of the endbulb- and calyx of Held-synapses in a mice model with Poisson-like firing before hearing onset (P2X2/P2X3Dbl-/-). The contribution of early auditory experience is investigated in a mouse model with conditional cell-specific hair cell ablation at different developmental stages (Pou4f3DTR/+). Experiments are designed for complementary use of in vivo- and slice-electrophysiology to assess functional processing through systemic acoustic stimulation and reveal underlying cellular mechanisms.

了解早期动作电位放电如何促进神经元网络的发育是发育神经生理学的主要挑战之一。在最初的发育过程之后，成熟神经元回路中的模式化的、不依赖于感觉的活动被认为指导精确感觉地图的形成。我们假设，不仅是活动，但模式prehearing活动，决定了巨大的听性脑干中的杯状突触的突触传递的发展。我们评估了听觉发作前泊松样放电小鼠模型（P2 X2/P2 X3 Db 1-/-）中Held突触的球内和萼内成熟的活动模式的作用。在不同发育阶段（Pou 4f 3DTR/+）具有条件性细胞特异性毛细胞消融的小鼠模型中研究早期听觉经验的贡献。实验的目的是补充使用体内和切片电生理学，以评估通过全身声刺激的功能处理，并揭示潜在的细胞机制。