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Regulatory architecture and variation of gene expression in wild and domesticated barley

野生和驯化大麦的调控结构和基因表达变异

基本信息

批准号：
281435309
负责人：
Dr. Martin Mascher, Ph.D.
金额：
--
依托单位：
Abteilung Genbank
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/281435309?language=en
关键词：
Regulatory architecture variation gene expression

项目摘要

Intraspecific variation of gene expression regulation in crop plants has been implicated in both domestication and adaptation to stress conditions. The recent advances in cereal genomics have enabled the genome-wide quantification of gene expression and the dissection of gene regulatory variation in diverse germplasm collections. The proposed research aims at ascertaining (i) whether barley domestication has been accompanied by a loss of regulatory variation and (ii) whether this entailed a concomitant reduction of variability in response to environmental perturbations. We will use high-throughput sequencing to quantify allele-specific expression in seedling tissue of four domesticated and four wild genotypes of barley and their F1 hybrids with a reference genotype to investigate to what extent domestication has reshaped the transcriptome of barley and how patterns of regulatory variation differ between wild and domesticated barley. We will also enquire into how abiotic stress modulates the regulation of gene expression in domesticated and wild barley by comparing expression data from seedlings grown under cold stress and control conditions. Our results will shed light at a genome-wide level on the relative contribution of cis- and trans-effects to gene regulation in barley, the inheritance of gene expression and their relation to genetic diversity and genome evolution. An improved understanding of the regulatory architecture of gene expression will contribute to the informed utilization of wild germplasm in crop improvement and to elucidating the molecular basis of heterosis in barley.

作物基因表达调控的种内变异与植物的驯化和逆境适应有关。谷物基因组学的最新进展使基因表达的全基因组定量和基因调控变异的解剖在不同的种质资源收集。拟议的研究旨在确定（i）大麦驯化是否伴随着监管变异的损失，以及（ii）这是否伴随着对环境扰动的变异性降低。我们将使用高通量测序来量化四个驯化和四个野生大麦基因型及其F1杂交种与参考基因型的幼苗组织中的等位基因特异性表达，以研究驯化在多大程度上重塑了大麦的转录组，以及野生大麦和驯化大麦之间的调控变异模式如何不同。我们还将探讨如何在驯化和野生大麦的非生物胁迫调制基因表达的调控，通过比较表达数据从幼苗生长在冷胁迫和控制条件下。我们的研究结果将揭示在全基因组水平上的相对贡献的顺式和反式效应的基因调控大麦，遗传基因表达及其与遗传多样性和基因组进化的关系。对大麦基因表达调控结构的深入了解将有助于野生种质在作物改良中的合理利用，并有助于阐明大麦杂种优势的分子基础。