Three-dimensional organization of chromatin across the plant cell cycle

植物细胞周期中染色质的三维组织

• 批准号: 391059743
• 负责人: Dr. Martin Mascher, Ph.D.
• 金额: --
• 依托单位: Abteilung Züchtungsforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/391059743?language=en
• 关键词: Three; dimensional; organization; chromatin; across

As the macromolecule complex that packages meters of genetic information in the micrometer-volume of a cell, chromatin have been under intense investigation for more than a century. Definite knowledge about the internal organization of human chromosomes and its relation to genome function has emerged recently, but much remains to be learned about the variation of chromatin folding along the cell cycle and between species. Methodological advances in cytological imaging and genomic assays have made it possible to interrogate chromatin contact probabilities and three-dimensional distances between genomic loci with high density and resolution. Our preliminary data on chromatin contact frequency at megabase resolution in barley mitotic chromosomes suggest fundamental differences in metaphase chromosome organization between Triticeae species and mammals. We propose to study chromatin folding throughout the cell cycle of barley using chromosome conformation capture sequencing, super-resolution microscopy, fluorescence in situ hybridization and computational polymer modeling. We will test whether chromatin adopts a regular solenoidal conformation in mitotic barley chromosomes. The proposed research will derive a experimentally validated structural model of chromosome organization in barley and elucidate conformational differences between large plant and smaller mammalian chromosomes.

一个多世纪以来，染色质作为一种大分子复合物，在细胞的微米体积中包裹着数米的遗传信息。关于人类染色体的内部组织及其与基因组功能的关系的明确知识最近已经出现，但关于染色质折叠在细胞周期和物种之间的变化仍有待了解。细胞学成像和基因组分析方法上的进步，使得高密度和高分辨率地询问染色质接触概率和基因组位点之间的三维距离成为可能。我们对大麦有丝分裂染色体的百万碱基分辨率染色质接触频率的初步数据表明，小麦和哺乳动物在中期染色体组织上存在根本差异。我们建议使用染色体构象捕获测序、超分辨率显微镜、荧光原位杂交和计算聚合物模型来研究大麦细胞周期中的染色质折叠。我们将测试在大麦有丝分裂染色体中染色质是否采用规则的螺线形构象。提出的研究将推导出一个实验验证的大麦染色体组织结构模型，并阐明大型植物和小型哺乳动物染色体之间的构象差异。

项目成果

Helical metaphase chromatid coiling is conserved

• DOI: 10.1101/2021.09.16.460607
• 发表时间: 2021-09
• 期刊: bioRxiv
• 作者: Ivona Kubalová;A. S. Câmara;P. Cápal;T. Beseda;J. Rouillard;G. Krause;Helena Toegelová;A. Himmelbach;N. Stein;A. Houben;J. Doležel;M. Mascher;H. Šimková;V. Schubert