Plant transformation: exploiting anti-apoptosis genes for very high efficiency transformation

植物转化：利用抗凋亡基因进行非常高效的转化

• 批准号: DP0773649
• 负责人: Prof James Dale
• 金额: $ 24.96万
• 依托单位: Queensland University of Technology
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2007
• 资助国家: 澳大利亚
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0773649
• 关键词: Plant; transformation; exploiting; anti; apoptosis

Crop improvement through genetic modification depends on the ability to transform target species. The most desirable method is Agrobacterium mediated transformation. However, plant species and cultivars differ significantly in their ability to be efficiently transformed by Agrobacterium. This is particularly true for the economically important cereals. We have discovered that anti-apoptosis genes, which inhibit programmed cell death, dramatically increase the Agrobacterium transformation efficiency in bananas and sugarcane. We will utilise this information and develop the use of these genes to increase the efficiency of transformation in those crops and cultivars that are difficult to transform using Agrobacterium.

通过转基因改良作物取决于转化目标物种的能力。目前最理想的转化方法是农杆菌介导法。然而，植物物种和栽培品种在其被农杆菌有效转化的能力上存在显著差异。对于具有重要经济意义的谷物来说，情况尤其如此。我们发现，抑制细胞程序性死亡的抗凋亡基因显著提高了农杆菌在香蕉和甘蔗中的转化效率。我们将利用这些信息并开发这些基因的使用，以提高那些用农杆菌难以转化的作物和品种的转化效率。