System-wide protein profiling along the mouse pain-axis during neuropathic pain by Data Independent Acquisition Mass Spectrometry (DIA-MS)

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Chronic Pain is a complex maladaptive disease that results from molecular changes occurring in different tissues of the nervous system involved in processing painful stimuli (pain-axis). However, associated system-wide alterations in protein networks along the pain-axis are currently largely unknown.The main aim of this proposal is to discover and validate significant protein changes occurring in seven different tissues of this pain-axis in a mouse model of neuropathic pain using emerging Data Independent Acquisition Mass Spectrometry (DIA-MS). Our preliminary work serves as a proof-of-principle of the potential of DIA-MS for discovering novel protein signatures of painful neuropathies in mice with unprecedented depth, reproducibility and sensitivity. We expect the here-proposed work program to significantly advance our understanding of neuropathic painful conditions with potentially far-reaching implications in pain management.