The hemolysin A secretion machinery from E. coli (A01)

大肠杆菌 (A01) 的溶血素 A 分泌机制

• 批准号: 289529939
• 金额: --
• 依托单位: Lehrstuhl für Biochemie I
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/289529939?language=en
• 关键词: hemolysin; secretion; machinery; E.; coli

We study a Type 1 secretion system (T1SS), which translocates the human pathogenic RTX toxin HlyA in one step across both membranes of E. coli. The project relates the dynamic assembly of the translocation complex with the molecular identity of the transport-competent components of this machinery. In a combined approach of in vitro and in vivo experiments the following key aspects will be addressed: (1) identification of switches and triggers involved in identity changes in T1SS (structural studies), (2) addressing the molecular interaction network and in vitro reconstitution of a T1SS (functional studies) and (3) analysis of cellular HlyA secretion and a detailed in vivo / in vitro correlation (cellular studies).

我们研究了一种1型分泌系统(T1SS)，它在一步中将人类致病性RTX毒素HlyA转移到大肠杆菌的两个膜上。该项目将转位复合体的动态组装与这一机械的运输能力组件的分子同一性联系起来。在体外和体内实验相结合的方法中，将解决以下关键方面：(1)识别参与T1SS身份变化的开关和触发器(结构研究)，(2)解决分子相互作用网络和T1SS的体外重建(功能研究)，(3)分析细胞HlyA分泌和详细的体内/体外相关性(细胞研究)。