アニオン性ヘテロキレート配位子を用いた複製可能な金属錯体型人工DNA塩基対の開発

使用阴离子杂螯合物配体开发可复制的金属复合物型人工DNA碱基对

• 批准号: 21J11332
• 负责人: HU Lingyun
• 金额: $ 0.96万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-28 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21J11332/
• 关键词: DNA; Artificial DNA; Metal-mediated base pair; Cu(II); DNA technology; Triazole-4-carboxylate; Artificial base pair; DNA nanotechnology

This study aims to develop suitable metal-mediated base pairs that can be enzymatically incorporated into the DNA. During my last year’s research, I have made several progresses as listed below:1.Designed and synthesized several novel nucleobases based on the criteria concluded from the previous research and obtained oligonucleotides containing one or more such base pairs. Apart from the ImOC, which was reported previously, the TazC is a novel candidate.2.Thermal melting analysis of DNA duplexes containing artificial nucleobases were conducted in the presence and absence of various metal ions, in which the TazC was found to form stable TazC-Cu(II)-TazC base pair.3.ESI-TOF MS measurements were conducted on the TazC base pair, and the formation of the Cu(II)-mediated TazC base pair was confirmed.

本研究旨在开发合适的金属介导的碱基对，可以通过酶促掺入DNA中。在过去一年的研究工作中，我取得了以下几个方面的进展：1.在前人研究的基础上，设计合成了几种新的碱基，并获得了含有一个或多个这样的碱基对的寡核苷酸。2.对含有人工碱基的DNA双链体在不同金属离子存在和不存在下进行了热熔融分析，发现TazC能形成稳定的TazC-Cu（II）-TazC碱基对。3.对TazC碱基对进行了ESI-TOF MS测量，并证实了Cu（II）介导的TazC碱基对的形成。

项目成果

Cu(II)-specific regulation of DNAzyme function through metal-mediated base pairing of carboxyimidazole-bearing nucleotides

通过金属介导的带有羧基咪唑的核苷酸的碱基配对对 DNAzyme 功能进行 Cu(II) 特异性调节

• 发表时间: 2021
• 作者: Hu Lingyun;Takezawa Yusuke;Nakama Takahiro;Shionoya Mitsuhiko
• 通讯作者: Shionoya Mitsuhiko

Metal-dependent activity regulation of a compact-sized DNAzyme containing an imidazole-4-carboxylate base pair

含有咪唑-4-羧酸盐碱基对的紧凑型脱氧核糖核酸酶的金属依赖性活性调节

• 发表时间: 2021
• 作者: Hu Lingyun;Takezawa Yusuke;Nakama Takahiro;Shionoya Mitsuhiko
• 通讯作者: Shionoya Mitsuhiko