Development of Artificial DNA-binding Protein Having Osmium Complex as Specific Methylcytosine Recongintion Unit

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To develop a new detection method of methylated DNA, an artificial protein has been developed that contains the zinc finger motif as a DNA-binding moiety as well as an osmium complex since the latter can bind preferentially to a methylcytosine rather than the unmethylated counterpart. Based on detailed investigation of osmium complexation with a methylcytosine, the novel protein has been synthesized via a solid-phase synthesis method and its binding ability toward methylated DNA molecules was evaluated.