Origin of Enzymes: Study of Hyperbranched Polymer Scaffolded Nanoparticles

我们开发了固态核磁共振（NMR）方法检测水结合和超支化聚合物内固定。根据结果，我们能够根据其水含量对不同的聚合物进行分类。在为特定反应设计催化剂的后期阶段，这种性质将变得重要。我们优化了缩肽超支化聚合物的合成和分析。该聚合物通过聚酯基质与氨基酸反应合成。我们设计了最佳的氨基酸混合物，允许可检测的选择而不压倒分析。我们更详细地研究了基于赖氨酸的超支化聚合物及其催化能力。我们发现多聚赖氨酸能够有效地催化肯普消除。

We developed solid-state nuclear magnetic resonance (NMR) methods for detecting water bound and immobilized inside hyperbranched polymers. Based on the results we were able to classify the different polymers based on their water content. This property will become important at the later stages when designing catalysts for specific reactions. We optimized synthesize and analyze depsipeptide hyperbranched polymers. The polymers are synthesized by reacting a polyester matrix with amino acids. We have devised optimal amino acid mixes that allow for detectable selection without overwhelming the analysis.We studied in more detail a lysine based hyperbranched polymer and it's catalytic ability. We found that polysine is capable of efficient catalysis of Kemp elimination.