Origin of Enzymes: Study of Hyperbranched Polymer Scaffolded Nanoparticles

我们开发了固体核磁共振（NMR）方法来检测超支化聚合物内部的水结合和固定化。根据测试结果，我们可以根据不同聚合物的含水量对其进行分类。在后期设计特定反应的催化剂时，这种性质将变得很重要。对沉积肽超支化聚合物进行了优化合成和分析。该聚合物是通过聚酯基质与氨基酸反应合成的。我们设计了最佳的氨基酸混合物，允许检测选择，而不会压倒分析。我们更详细地研究了赖氨酸基超支化聚合物及其催化性能。我们发现多聚氨酸能够有效地催化Kemp的消除。

We developed solid-state nuclear magnetic resonance (NMR) methods for detecting water bound and immobilized inside hyperbranched polymers. Based on the results we were able to classify the different polymers based on their water content. This property will become important at the later stages when designing catalysts for specific reactions. We optimized synthesize and analyze depsipeptide hyperbranched polymers. The polymers are synthesized by reacting a polyester matrix with amino acids. We have devised optimal amino acid mixes that allow for detectable selection without overwhelming the analysis.We studied in more detail a lysine based hyperbranched polymer and it's catalytic ability. We found that polysine is capable of efficient catalysis of Kemp elimination.