Origin of Enzymes: Study of Hyperbranched Polymer Scaffolded Nanoparticles

酶的起源：超支化聚合物支架纳米颗粒的研究

• 批准号: 21K03735
• 负责人: ママジャノフ イリーナ
• 金额: $ 2.58万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K03735/
• 关键词: hyperbranched polymer; polyester; wet-dry cycle; smart material; protoenzyme; biomimetic catalysis; chemical evolution; origin of life; nanoparticle; biomimetic catalyst

We developed solid-state nuclear magnetic resonance (NMR) methods for detecting water bound and immobilized inside hyperbranched polymers. Based on the results we were able to classify the different polymers based on their water content. This property will become important at the later stages when designing catalysts for specific reactions. We optimized synthesize and analyze depsipeptide hyperbranched polymers. The polymers are synthesized by reacting a polyester matrix with amino acids. We have devised optimal amino acid mixes that allow for detectable selection without overwhelming the analysis.We studied in more detail a lysine based hyperbranched polymer and it's catalytic ability. We found that polysine is capable of efficient catalysis of Kemp elimination.

我们开发了固体核磁共振(核磁共振)方法来检测超支化聚合物中的水结合和固定。根据结果，我们能够根据不同聚合物的水分含量对其进行分类。在为特定反应设计催化剂的后期阶段，这一特性将变得重要。我们优化合成并分析了去脂肽超支化聚合物。聚合物是由聚酯基质与氨基酸反应合成的。我们设计了最佳的氨基酸组合，允许在不超过分析的情况下进行可检测的选择。我们更详细地研究了一种基于赖氨酸的超支化聚合物及其催化能力。我们发现，多氨酸能够有效地催化Kemp消除。

项目成果

Single-molecule origin of life scenario: Necessity and feasibility

单分子生命起源场景：必要性和可行性

• DOI: 10.1016/j.plrev.2021.04.007
• 发表时间: 2021
• 期刊: Physics of Life Reviews
• 影响因子: 11.7
• 作者: Haotian Liu;Nobutatsu Mochizuki;Chie Kato;Tesfaye Kidane;Ameha Muluneh;Masakazu Fujii;Ryokei Yoshimura;Shin-ichi Kagashima;Yo-ichiro Otofuji;Naoto Ishikawa;Mamajanov Irena
• 通讯作者: Mamajanov Irena

Incorporation of Basic α-Hydroxy Acid Residues into Primitive Polyester Microdroplets for RNA Segregation

• DOI: 10.1021/acs.biomac.0c01697
• 发表时间: 2021-03-05
• 期刊: BIOMACROMOLECULES
• 影响因子: 6.2
• 作者: Jia, Tony Z.;Bapat, Niraja, V;Chandru, Kuhan
• 通讯作者: Chandru, Kuhan