Potential new enzymes in plant NO biosynthesis

植物一氧化氮生物合成中潜在的新酶

• 批准号: 29119983
• 负责人: Professor Dr. Günther Scherer, Ph.D.
• 金额: --
• 依托单位: Institut für Gartenbauliche Produktionssysteme
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/29119983?language=en
• 关键词: Potential; new; enzymes; plant; NO

This project aims at identifying the source enzyme(s) of polyamine-induced nitric oxide (NO). Polyamine-induced NO synthesis was discovered in the lab of the PI and is unprecedented. In contrast to published information about plant NO synthesizing enzymes, exogenous arginine is less effective than polyamines (putrescine, spermine, spermidine) in inducing rapid NO biosynthesis. As polyamine induction of NO synthesis is very rapid this indicates that perhaps known enzymes of plant NO biosynthesis or a new enzyme group for NO biosynthesis use polyamines as a substrate. The search will encompass the known AtNOS gene family and the polyamine oxidase gene family. The protein identified will be recombinantly purified and tested as a NO synthase and expressed under a chemically controlled promoter in Arabidopsis so that changes in NO biosynthesis and phenotype can be demonstrated. As polyamines have a wide-spread physiological significance in plants the project will give a new foundation to polyamine biology in plants.

本项目旨在确定多胺诱导的一氧化氮(NO)的来源酶(S)。在PI的实验室中发现了多胺诱导的NO合成，这是史无前例的。与已发表的关于植物NO合成酶的信息相比，外源精氨酸在诱导快速NO生物合成方面不如多胺(腐胺、精胺、亚精胺)有效。由于多胺对NO合成的诱导非常迅速，这表明可能已知的植物NO生物合成酶或一个新的NO生物合成酶基团使用多胺作为底物。搜索将包括已知的AtNOS基因家族和多胺氧化酶基因家族。鉴定出的蛋白质将被重组纯化并作为一氧化氮合酶进行检测，并在拟南芥中的化学控制启动子下表达，以便观察一氧化氮合酶生物合成和表型的变化。由于多胺在植物中具有广泛的生理意义，该项目将为植物多胺生物学奠定新的基础。