Functions of patatin-related phospholipases A in auxin and light signalling

patatin 相关磷脂酶 A 在生长素和光信号传导中的功能

• 批准号: 104138357
• 负责人: Professor Dr. Günther Scherer, Ph.D.
• 金额: --
• 依托单位: Institut für Gartenbauliche Produktionssysteme
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/104138357?language=en
• 关键词: Functions; patatin; related; phospholipases; auxin

The functions of the gene family of the 10 AtPLA genes in Arabidopsis will be investigated. Previous data indicate functions in auxin and pathogen signalling, new data indicate additional functions in light signalling. In particular is planned: Analysis of promoter::GUS plants and subcellular localization by GFP-pPLA hybrid protein expression, analysis of morphological responses of knockout lines to auxin, light and other signals and stresses (induced phenotypes). The analysis of functional linkage of AtPLA genes specifically to auxin or light will be done by transcription analysis of genes rapidly regulated by signal prior to the expression of any new protein (<30-45 min). Light activation (blue, red, far red) of pPLA activity will be investigated by analysis of lipid metabolites. Crosses of relevant knockouts/mutants with functions in similar signalling networks will be generated to obtain stronger phenotypes and double/triple mutants will be analyzed.

本研究将对拟南芥中这10个AtPLA基因家族的功能进行研究。以前的数据表明生长素和病原体信号的功能，新的数据表明光信号的额外功能。特别是计划：启动子的分析：GUS植物和通过GFP-pPLA杂合蛋白表达的亚细胞定位，敲除株系对生长素、光和其它信号和胁迫（诱导的表型）的形态学响应的分析。AtPLA基因特异性与生长素或光的功能性连锁的分析将通过在任何新蛋白质表达之前（<30-45分钟）由信号快速调节的基因的转录分析来进行。将通过分析脂质代谢物研究pPLA活性的光活化（蓝色、红色、远红色）。将产生在类似信号传导网络中具有功能的相关敲除/突变体的杂交，以获得更强的表型，并将分析双/三突变体。