Nanoscale probing and visualization of Annexin A5 assembly, function, and dynamics on native cellular membranes

该项目的目的是了解不同的生物物理和生化参数如何调节膜联蛋白A5超分子组装，并最终可视化来自分离细胞的天然膜上的膜联蛋白阵列。我们获得的初步数据表明，膜流动性是一个关键的驱动程序调节膜联蛋白多晶晶格的形成和晶体结构。利用荧光成像和高速原子力显微镜对模型膜（平面人工双层膜）进行了初步的实验观察。光开关化合物也被用来操纵膜的性质，并证明膜联蛋白超分子多态性。

The aim of this project was to understand how different biophysical and biochemical parameters regulate annexin A5 supramolecular assembly and ultimately visualize annexin arrays on native membranes from isolated cells. We obtained preliminary data demonstrating that membrane fluidity is a key driver regulating annexin polycrystalline lattice formation and crystallographic configuration. The inital experimental observations were carried out on model membranes (planar artificial bilayer)by means of fluorescence imaging and high-speed atomic force microscopy. Photoswitchable compounds were also used to manipulate membrane properties and demonstrate annexin supramolecular polymorphism.