哺乳類のDNA複製タイミング制御におけるゲノムとエピゲノムの機能的リンク

基因组和表观基因组在控制哺乳动物 DNA 复制时间方面的功能联系

• 批准号: 21K06129
• 负责人: SHARIF JAFAR
• 金额: $ 2.66万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K06129/
• 关键词: Euchromatin; Heterochromatin; Replication timing; A/B compartments; Transcription; Epigenome; DNA replication; H2B monoubiquitination; RNF20; Retrotransposon

The eukaryotic genome is organized into eu- or hetero-chromatin domains, the former is gene-rich and transcriptionally active, while the latter is gene-poor and transcriptionally inactive. Eu- or hetero-chromatin are also analogous to A or B compartments, calculated from the PC1 (primary component 1) value of the eigenvector of the Hi-C (a method for chromatin conformation capture) matrix. During S phase, A compartments replicate early while B compartments replicate late. It is, however, not understood if A/B compartmentalization and early/late replication timing is mechanistically linked.

真核生物的基因组被组织成真染色质或异染色质结构域，前者是基因丰富和转录活性，而后者是基因贫乏和转录失活。Eu-或异染色质也类似于A或B区室，根据Hi-C（染色质构象捕获方法）矩阵的特征向量的PC 1（主要成分1）值计算。在S期，A区室复制早，而B区室复制晚。然而，目前还不清楚A/B区室化和早期/晚期复制时间是否存在机械联系。

项目成果

Efficient de novo assembly and modification of large DNA fragments

大 DNA 片段的高效从头组装和修饰

• DOI: 10.1007/s11427-021-2029-0
• 发表时间: 2021-12-16
• 期刊: SCIENCE CHINA-LIFE SCIENCES
• 影响因子: 9.1
• 作者: Jiang, Shuangying;Tang, Yuanwei;Dai, Junbiao
• 通讯作者: Dai, Junbiao

Bridging multiple dimensions: roles of transposable elements in higher-order genome regulation

• DOI: 10.1016/j.gde.2023.102035
• 发表时间: 2023-04-05
• 期刊: CURRENT OPINION IN GENETICS & DEVELOPMENT
• 影响因子: 4
• 作者: Sharif，Jafar;Koseki，Haruhiko;Parrish，Nicholas F.
• 通讯作者: Parrish，Nicholas F.