In vivo imaging of endocannabinoid-regulated neuronal network activity coupled with 3D movement analysis in freely behaving mice

内源性大麻素调节的神经元网络活动的体内成像与自由行为小鼠的 3D 运动分析相结合

• 批准号: 21K06399
• 负责人: Ignatowska・Jankowska Bogna
• 金额: $ 2.66万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K06399/
• 关键词: Endocannabinoid system; Movement kinematics; Neuronal activity; Mouse behavior; 3D Motion capture; Calcium imaging; 3D motion capture; neuronal activity; movement kinematics; endocannabinoid system; mouse behavior; olivocerebellar system; endo

This phase of the project was devoted to conducting behavioral experiments and data acquisition. In the fiscal year 2022, we implanted miniature endoscopes to cerebellar nuclei and successfully recorded neuronal network activity using calcium imaging simultaneously with the recording of high-resolution 3D movement trajectories during complex behaviors in freely moving mice. We have also conducted preliminary experiments that will allow similar in vivo imaging of the inferior olive. These experiments allow us to combine the neuronal activity of large populations with a high-precision recording of movement kinematics which will enable the unraveling of neurobiological mechanisms underlying coordinated behaviors.

这一阶段的项目致力于进行行为实验和数据采集。在2022财年，我们将微型内窥镜植入小脑核，并成功地使用钙成像记录了自由运动小鼠复杂行为时的神经元网络活动，同时记录了高分辨率的3D运动轨迹。我们还进行了初步的实验，将允许类似的下橄榄的体内成像。这些实验使我们能够将大量人群的神经元活动与运动运动学的高精度记录结合起来，这将使我们能够解开协调行为背后的神经生物学机制。

项目成果

Distinct kinematic characteristics of MAGL and FAAH inhibition in high precision 3D motion capture analysis of freely behaving mice

自由行为小鼠高精度 3D 运动捕捉分析中 MAGL 和 FAAH 抑制的独特运动学特征

• 发表时间: 2021
• 作者: 服部 剛志,Roboon Jureepon;堀 修;Bogna Ignatowska-Jankowska
• 通讯作者: Bogna Ignatowska-Jankowska