In vivo imaging of endocannabinoid-regulated neuronal network activity coupled with 3D movement analysis in freely behaving mice

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This phase of the project was devoted to conducting behavioral experiments and data acquisition. In the fiscal year 2022, we implanted miniature endoscopes to cerebellar nuclei and successfully recorded neuronal network activity using calcium imaging simultaneously with the recording of high-resolution 3D movement trajectories during complex behaviors in freely moving mice. We have also conducted preliminary experiments that will allow similar in vivo imaging of the inferior olive. These experiments allow us to combine the neuronal activity of large populations with a high-precision recording of movement kinematics which will enable the unraveling of neurobiological mechanisms underlying coordinated behaviors.