In vivo imaging of endocannabinoid-regulated neuronal network activity coupled with 3D movement analysis in freely behaving mice

这一阶段的项目致力于进行行为实验和数据采集。在2022财年，我们将微型内窥镜植入小脑核，并成功地使用钙成像记录了自由运动小鼠复杂行为时的神经元网络活动，同时记录了高分辨率的3D运动轨迹。我们还进行了初步的实验，将允许类似的下橄榄的体内成像。这些实验使我们能够将大量人群的神经元活动与运动运动学的高精度记录结合起来，这将使我们能够解开协调行为背后的神经生物学机制。

This phase of the project was devoted to conducting behavioral experiments and data acquisition. In the fiscal year 2022, we implanted miniature endoscopes to cerebellar nuclei and successfully recorded neuronal network activity using calcium imaging simultaneously with the recording of high-resolution 3D movement trajectories during complex behaviors in freely moving mice. We have also conducted preliminary experiments that will allow similar in vivo imaging of the inferior olive. These experiments allow us to combine the neuronal activity of large populations with a high-precision recording of movement kinematics which will enable the unraveling of neurobiological mechanisms underlying coordinated behaviors.