DNA-Metabarcoding of phyto- and zooplankton in East African lake sediments as proxies for past environmental perturbation

东非湖泊沉积物中浮游植物和浮游动物的 DNA 宏条形码作为过去环境扰动的代表

• 批准号: 299025851
• 负责人: Professor Dr. Michael Hofreiter
• 金额: --
• 依托单位: Institut für Biochemie und Biologie
• 依托单位国家: 德国
• 项目类别: Infrastructure Priority Programmes
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/299025851?language=en
• 关键词: DNA; Metabarcoding; phyto; zooplankton; East

Lake-sediment cores provide natural archives of past environmental changes, traditionally analyzed with sedimentological, geochemical and paleontological methods. More recently, samples from sediment cores have also been subjected to molecular DNA analysis, targeting either the living community of soil microbes or remnants of organisms that inhabited the lake and/or its surface sediment in the past. The project proposed here aims at evaluating the possibility for DNA metabarcoding in the Chew Bahir sediment cores, combining state-of-the-art techniques of environmental genomics and ancient DNA analysis. The principal research questions addressed by the project are: (1) How far back in time can DNA remnants in the Chew Bahir sediment cores be extracted and analyzed? (2) How congruent are the results of PCR-based vs. hybridization-capture-based metabarcoding? and (3) What are the long-term trends and shifts in the plankton communities in the Chew Bahir record? The results of this project will provide an important contribution to the understanding of interactions between the environment and the biosphere in the past, particularly in sediments, in which microscopically recognizable organic remains are scarce or absent. Furthermore, we expect important methodological advances in the study of sediments of tropical lakes, which will be in future projects of great importance.

湖泊沉积物岩心提供了过去环境变化的自然档案，传统上采用沉积学、地球化学和古生物学方法进行分析。最近，沉积物岩心样本也接受了分子 DNA 分析，目标是土壤微生物的活群落或过去居住在湖泊和/或其表面沉积物中的生物体残余物。这里提出的项目旨在评估 Chew Bahir 沉积岩芯中 DNA 元条形码的可能性，结合最先进的环境基因组学和古代 DNA 分析技术。该项目解决的主要研究问题是：(1) 可以提取和分析 Chew Bahir 沉积岩芯中的 DNA 残留物多久？ (2) 基于 PCR 的元条形码与基于杂交捕获的元条形码的结果有多一致？ (3) Chew Bahir 记录中浮游生物群落的长期趋势和变化是什么？该项目的结果将为了解过去环境与生物圈之间的相互作用做出重要贡献，特别是在沉积物中，在沉积物中，微观可识别的有机遗骸很少或不存在。此外，我们预计热带湖泊沉积物研究的方法论将取得重要进展，这将在未来的项目中发挥重要作用。