Impact of Invasion and Environmental Change upon the Microbiome of an Algal Holobiont

入侵和环境变化对藻类 Holobiont 微生物组的影响

• 批准号: 299367406
• 负责人: Dr. Till Bayer, Ph.D.
• 金额: --
• 依托单位: GEOMAR Helmholtz-Zentrum für Ozeanforschung Kiel
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/299367406?language=en
• 关键词: Impact; Invasion; Environmental; Change; upon

Holobionts require the functional support of associated microorganisms and the required functions are primarily determined by missing functions of the host. At the same time certain associated microorganisms such as opportunistic pathogens may also feature functions that could be detrimental to the host. A functional holobiont therefore requires a stable equilibrium of functions. Transportation into a new and different environment could obviously disturb this equilibrium, for example if important components of the associated microbial community cannot survive.On the other hand in the new environment non-native hosts may be confronted with microbes that feature new combinations of beneficial and detrimental functions. Such challenges could drive a selection of host individuals that have a special capacity for the formation of a stable equilibrium of functions with the new microepibiont community. These important aspects of holobiont invasion biology are nearly unstudied and will be studied for the first time with a macroalgal host, Gracilaria vermiculophylla, in this project. The species is considered as one of the more problematic marine invasive macroalgae and an urgent necessity to understand the determinants of this success is apparent. Moreover, G. vermiculophylla is currently the only invasive seaweed that fulfills an important precondition for common garden experiments: an established protocol for sampling and transportation on a global scale without loss of host individuals exists. A first objective is to investigate whether (a) any bacterial taxa are consistently associated with this algal host at locations in its native range (Asia) and in its non-native range (Europe and the American East and West coasts) and whether (b) any functions are consistently overrepresented in those associations. If this is the case then the identified taxa or functions could represent a core microbiome that is necessary for long term survival and invasion success of the host. In a second step we will follow the taxonomic and functional reassembly of disturbed microbiomes on host individuals that originate from native and non-native Gracilaria populations and are exposed to identical pools of settlers in common gardens. This approach will allow it to evaluate the interactive effect of settler availability and environment upon the microbiome assembly (objective 2) and to detect any differences in host-microbiome interactions among the different algal populations (objective 3).

Holobion需要相关微生物的功能支持，而所需的功能主要由宿主缺失的功能决定。与此同时，某些相关微生物，如机会性病原体，也可能具有对宿主有害的功能。因此，功能全息图需要稳定的功能平衡。运输到新的和不同的环境中显然会扰乱这种平衡，例如，如果相关微生物群落的重要组成部分无法生存。另一方面，在新的环境中，非本地宿主可能面临具有新的有益和有害功能组合的微生物。这样的挑战可能会促使一批具有特殊能力的寄主个体与新的微生物群落形成稳定的功能平衡。海藻入侵生物学的这些重要方面几乎没有被研究过，在这个项目中，将首次用大型藻类宿主Gracilaria vermiculophylla进行研究。该物种被认为是问题较多的海洋入侵大型藻类之一，显然迫切需要了解这一成功的决定因素。此外，蠕藻是目前唯一的入侵海藻，它满足了普通花园实验的一个重要前提条件：存在一个在全球范围内进行采样和运输而不损失宿主个体的既定协议。第一个目标是调查(A)是否有任何细菌分类群在其原生范围(亚洲)和非原生范围(欧洲和美洲东西海岸)的位置与该藻类宿主一致地联系在一起，以及(B)是否有任何功能在这些联系中持续过多地被代表。如果是这样的话，已确定的分类群或功能可能代表了寄主长期生存和入侵成功所必需的核心微生物群。在第二步中，我们将跟踪寄主个体上受干扰的微生物群落的分类和功能重组，这些寄主个体来自本地和非本地江豚种群，并暴露在共同花园中相同的定居者池中。这一方法将使其能够评估定居者的可获得性和环境对微生物组组装的交互影响(目标2)，并检测不同藻类之间宿主-微生物组相互作用的任何差异(目标3)。

项目成果

The Use of Photographic Color Information for High-Throughput Phenotyping of Pigment Composition in Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N.Norris & Fredericq

使用摄影颜色信息对蛰海藻色素组合物进行高通量表型分析 Gurgel，J N Norris

• DOI: 10.5252/cryptogamie-algologie2019v40a7
• 发表时间: 2019
• 期刊: Cryptogamie, Algologie
• 作者: W.H. Ryan;S. Heiser;M.D. Curtis;C.D. Amsler;T. Bayer;G. Bonthond;G. Wang;F. Weinberger;S.A. Krueger-Hadfield
• 通讯作者: S.A. Krueger-Hadfield

How do microbiota associated with an invasive seaweed vary across scales?

• DOI: 10.1111/mec.15470
• 发表时间: 2020-06-13
• 期刊: MOLECULAR ECOLOGY
• 影响因子: 4.9
• 作者: Bonthond, Guido;Bayer, Till;Weinberger, Florian
• 通讯作者: Weinberger, Florian