Forward programming human induced pluripotent stem cells into chondrocytes by forced expression of SOX9

通过强制表达 SOX9 将人诱导多能干细胞正向编程为软骨细胞

• 批准号: 299467474
• 负责人: Privatdozentin Dr. Solvig Diederichs
• 金额: --
• 依托单位: Klinik für Orthopädie und Unfallchirurgie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/299467474?language=en
• 关键词: Forward; programming; human; induced; pluripotent

Human induced pluripotent stem cells (hiPSCs) are highly attractive for cartilage regenerative medicine, because they can be derived autologously in potentially unlimited amounts and comprise the inherent capacity to give rise to stable hyaline cartilage. Currently, there is no standard strategy to induce chondrogenic differentiation of hiPSCs, and the majority of proposed methods suffer from either low efficiency or are enormously laborious, thus impairing their applicability for regenerative medicine. In this project, hiPSCs will be directly induced into chondrocytes by genetic modification. This forward programming promises to considerably simplify hiPSC chondrogenesis by circumventing the complex regulatory networks of multistage hiPSC differentiation. Aim of this project is to generate high-quality cartilage from hiPSCs that meets the high quality standards set by human autologous chondrocytes that represent the current gold standard.

人类诱导多能干细胞（hiPSC）对于软骨再生医学非常有吸引力，因为它们可以无限量地自体衍生，并且具有产生稳定透明软骨的固有能力。目前，还没有诱导 hiPSC 软骨形成的标准策略，并且大多数提出的方法要么效率低，要么非常费力，从而削弱了它们在再生医学中的适用性。在这个项目中，hiPSC将通过基因修饰直接诱导成软骨细胞。这种正向编程有望通过绕过多阶段 hiPSC 分化的复杂调控网络来显着简化 hiPSC 软骨形成。该项目的目标是从 hiPSC 中生成高质量软骨，满足代表当前黄金标准的人类自体软骨细胞制定的高质量标准。

项目成果

Ein initialer WNT-Puls verbessert Zellüberleben und Gewebeausbeute während der iPS-Chondrogenese

初始 WNT 脉冲可提高 iPS 软骨形成过程中的细胞存活率和组织产量

• DOI: 10.1055/s-0040-1717236
• 发表时间: 2020
• 期刊: Zeitschrift für Orthopädie und Unfallchirurgie
• 作者: Kreuser U;Richter W;Diederichs S
• 通讯作者: Diederichs S

Cells for Cartilage Regeneration

软骨再生细胞

• DOI: 10.1007/978-3-319-37076-7_1-1
• 发表时间: 2018
• 期刊: Cell Engineering and Regeneration
• 作者: van Osch GJVM;Barbero A;Brittberg M;Correa D;Diederichs S;Goldring MB
• 通讯作者: Goldring MB

Chondral Differentiation of Induced Pluripotent Stem Cells Without Progression Into the Endochondral Pathway

• DOI: 10.3389/fcell.2019.00270
• 发表时间: 2019-11-01
• 期刊: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
• 影响因子: 5.5
• 作者: Diederichs, Solvig;Klampfleuthner, Felicia A. M.;Richter, Wiltrud
• 通讯作者: Richter, Wiltrud

Significance of MEF2C and RUNX3 Regulation for Endochondral Differentiation of Human Mesenchymal Progenitor Cells

• DOI: 10.3389/fcell.2020.00081
• 发表时间: 2020-03-04
• 期刊: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
• 影响因子: 5.5
• 作者: Dreher, Simon, I;Fischer, Jennifer;Richter, Wiltrud
• 通讯作者: Richter, Wiltrud

The Role of Extracellular Matrix Expression, ERK1/2 Signaling and Cell Cohesiveness for Cartilage Yield from iPSCs

• DOI: 10.3390/ijms20174295
• 发表时间: 2019-09-01
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
• 影响因子: 5.6
• 作者: Buchert, Justyna;Diederichs, Solvig;Richter, Wiltrud
• 通讯作者: Richter, Wiltrud