The interplay between specificity and stability in lactamases: molecular modeling of flexibility and dynamics

内酰胺酶特异性和稳定性之间的相互作用：灵活性和动力学的分子模型

• 批准号: 30347923
• 负责人: Professor Dr. Jürgen Pleiss
• 金额: --
• 依托单位: Abteilung für Technische Biochemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/30347923?language=en
• 关键词: interplay; between; specificity; stability; lactamases

In the proposed project we develop a general molecular model to describe and predict how structure, flexibility, and dynamics of serine lactamases is modified by destabilizing mutations that improve specificity, thus transferring extended spectrum lactamase activity or inhibitor resistance, and mutations that increase stability, but have no effect to activity. Our working hypothesis is that destabilization and stabilization could result from either of two mechanism: from well-known local effects like packing, electrostatics, hydrogen bonding, and solvent effects, or from long-range effects like increased backbone flexibility and coupling of motions, as it has been suggested only recently. We will study these mechanisms by massive multiple molecular dynamics simulations and de novo protein design tools. Understanding the interplay of destabilizing and stabilizing mutations would help to understand natural evolution and provide a design strategy which could be generally used in protein engineering. Predictions of our model are validated by a co-operation with three experimental partners: (1) Detection of new stabilizing mutants by directed evolution and biochemical characterization of engineered mutants (Prof. Dr. Kristian Müller, Freiburg, in the framework of SPP 1170). (2) If promising, an NMR study of protein backbone flexibility will be carried out (Dr. Jörn Werner, University of Southampton) (3) Analysis of lactamase variants isolated from clinical samples (PD Dr. Till Bachmann, Institute of Technical Biochemistry, Stuttgart).

在拟议的项目中，我们开发了一个通用的分子模型来描述和预测丝氨酸内酰胺酶的结构、灵活性和动力学是如何被不稳定突变所改变的，这些突变提高了特异性，从而转移了广谱内酰胺酶活性或抑制剂抗性，以及增加稳定性但对活性没有影响的突变。我们的工作假设是，不稳定和稳定可能来自两种机制中的任何一种：来自众所周知的局部效应，如填料、静电、氢键和溶剂效应，或者来自远程效应，如骨干柔韧性的增加和运动的耦合，这是最近才提出的。我们将通过大规模的多分子动力学模拟和从头开始的蛋白质设计工具来研究这些机制。了解不稳定突变和稳定突变的相互作用将有助于理解自然进化，并提供一种可用于蛋白质工程的设计策略。通过与三个实验伙伴的合作，我们的模型预测得到了验证：(1)通过定向进化和工程突变体的生化表征检测新的稳定突变体（Freiburg的Kristian m<s:1> ller教授，在SPP 1170的框架内）。(2)如果有希望，将进行蛋白质骨架柔韧性的核磁共振研究（Jörn Werner博士，南安普敦大学）(3)从临床样品中分离的内酰胺酶变异分析（斯图加特技术生物化学研究所PD博士Till Bachmann）。