The nature and roles of cellular metabolism and energy in the regulation of neuronal chain migration

细胞代谢和能量在神经元链迁移调节中的性质和作用

• 批准号: 20K06865
• 负责人: ZHU YAN
• 金额: $ 2.83万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K06865/
• 关键词: metabolic pathway; neuronal migration; bioenergetics; ATP sensor; Bioenergetics; metabolic pathways; Neuronal migration; chain migration; energy status; energy sensor

This research has two objectives: (1) visualize and quantify the energy status of chain migrating neurons; (2) unravel the metabolic sources that fuel neuronal migration.For objective (1), while I have established a system to express genetically encoded ATP sensors (ATeam) in vivo and in vitro, progress in FRET-based imaging of intracellular ATP is hindered by technical difficulties. For objective (2), I have generated a mouse knockout line with deficiency in a transcription factor known to promote OXPHOS metabolic pathway during development. Phenotypic analysis of the mutant line has shown defects in neuronal migration and/or their termination of migration.

本研究有两个目的：（1）可视化和量化链迁移神经元的能量状态;（2）揭示驱动神经元迁移的代谢来源。 对于目标（2），我已经产生了一种小鼠敲除系，其具有已知在发育期间促进OXPHOS代谢途径的转录因子的缺陷。突变株的表型分析显示神经元迁移和/或其迁移终止的缺陷。

项目成果

NHLH-1 & -2, a pair of highly related bHLH transcriptional factors, synergistically control the expression of Robo3 in mouse precerebellar neurons

NHLH-1

• 发表时间: 2021
• 作者: Yuki Setoguchi;Akiko Hayashi;Ayami Kawada;Ayako Ibusuki;Daigo Yanaoka;Ryota Saito;Tomoko Ishibashi;Hiroaki Takimoto;Yoshihide Yamaguchi;Hirokazu Ohtaki;and Hiroko Baba;吉村 武;Yan Zhu
• 通讯作者: Yan Zhu

Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation

趋化因子受体CXCR7非细胞自主控制脑桥神经元迁移和细胞核形成

• DOI: 10.1038/s41598-020-68852-z
• 发表时间: 2020
• 期刊: Scientific Reports
• 影响因子: 4.6
• 作者: Zhu Yan;Hirata Tatsumi;Mackay Fabienne;Murakami Fujio
• 通讯作者: Murakami Fujio